NDLI: The Effect of Photoautotrophy on Photosynthesis and Photoinhibition of Gardenia Plantlets during Micropropagation

Content Provider	Springer Nature Link
Author	Serret, M.D. Trillas, M.I. Matas, J. Araus, J.L.
Copyright Year	2001
Abstract	We studied the relationships between the degree of photoautotrophy, photosynthetic capacity, and extent of photoinhibition of Gardenia jasminoides Ellis plantlets in vitro. Two successive micropropagation stages (shoot multiplication and root induction), and three culture conditions [tube cap closure, photosynthetic photon flux density (PPFD), and sucrose concentration] which may influence the development of photoautotrophy in vitro were assayed. The ratios of variable chlorophyll fluorescence to either maximal (F$_{v}$/F$_{m}$) or ground (F$_{v}$/F$_{0}$) values were low, irrespective of the culture stage or growing conditions. Incomplete development of the photosynthetic apparatus and permanent photoinhibition may be involved. However, F$_{v}$/F$_{m}$ and F$_{v}$/F$_{0}$ increased from shoot multiplication to root induction owing to a decrease in F$_{0}$ and an increase in F$_{m}$. This suggests that photoinhibition decreases later during micropropagation, when the photoautotrophy of plantlets is more advanced. The low sucrose content and high PPFD increased the photoinhibition of plantlets, whereas growth in tubes with permeable caps showed the opposite effect. The only culture factor with a significant (positive) effect on maximum photosynthetic rate (P $_{max}$) was PPFD. At shoot multiplication net photosynthetic rate (P $_{N}$) was positively correlated with the half time of the increase from F$_{0}$ to F$_{m}$ (t$_{1/2}$). Such association may be mainly due to a common response of both traits to higher PPFD in culture. Within each culture stage, no relationship was observed between P $_{N}$ and the degree of photoautotrophy, which was positively correlated with F$_{v}$/F$_{m}$ and F$_{v}$/F$_{0}$ during root induction. During shoot multiplication, these correlations were not significant, or were even negative. Hence during the last stage of micropropagation, plantlets with a higher degree of photoautotrophy are less photoinhibited, whereas they do not follow this pattern at the earlier stage.
Starting Page	245
Ending Page	255
Page Count	11
File Format	PDF
ISSN	03003604
Journal	Photosynthetica
Volume Number	39
Issue Number	2
e-ISSN	15739058
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2001-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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