NDLI: Purification and Some Catalytic Properties of Glucose-6-Phosphate Dehydrogenase Isoforms from Barley Leaves

Content Provider	Springer Nature Link
Author	Semenihina, A. Popova, T. Schnarrenberger, C. Pinheiro De Carvalho, M.A.A.
Copyright Year	2001
Abstract	The cytosolic and chloroplastic isoforms of glucose-6-phosphate dehydrogenase (G$_{6}$PDH) were separated and purified from barley leaves (Hordeum vulgare L.). In etiolated leaves, only the cytosolic isoform was expressed. The molecular mass of the cytosolic enzyme, G$_{6}$PDH$_{1}$, was 112±8 kDa and that of the chloroplast enzyme, G$_{6}$PDH$_{2}$, was 136±7 kDa. The K$_{m}$ values for glucose-6-phosphate and NADP were 0.133 and 0.041 mM for G$_{6}$PDH$_{1}$, and 0.275 and 0.062 mM for G$_{6}$PDH$_{2}$, respectively. The pH optimum was 8.2 for G$_{6}$PDH$_{1}$ and 7.8 for G$_{6}$PDH$_{2}$. The enzyme is absolutely specific for NADP. NADPH is a competitive inhibitor of the G$_{6}$PDH$_{1}$ in respect to glucose-6-phosphate (G$_{6}$P) and NADP (K$_{i}$ = 0.050 and 0.025 mM, respectively). NADPH is a competitive inhibitor of the G$_{6}$PDH$_{2}$ in respect to NADP (K$_{i}$ = 0.010 mM), but a non-competitive inhibitor in respect to the G$_{6}$P. ADP, AMP, UTP, NAD, and NADH had no effect on the activity of G$_{6}$PDH. ATP inhibited the G$_{6}$PDH$_{2}$ activity.
Starting Page	299
Ending Page	304
Page Count	6
File Format	PDF
ISSN	03003604
Journal	Photosynthetica
Volume Number	39
Issue Number	2
e-ISSN	15739058
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2001-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Glucose-6-phosphate dehydrogenase in barley roots: kinetic properties and localisation of the isoforms

Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties

Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties

Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties

PURIFICATION AND PROPERTIES OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM CORIANDER (CORIANDRUM SATIVUM) LEAVES

Purification and Characteristics of Sorbitol-6-phosphate Dehydrogenase from Loquat Leaves 1

Glucose-6-phosphate Dehydrogenase from Agaricus bispovus : Purification and Properties

Purification and Properties of Microsomal Glucose 6-Phosphate Dehydrogenase ( Hexose 6-Phosphate Dehydrogenase ) of Rat Liver

Glucose 6-Phosphate Dehydrogenase Isozymes of Maize Leaves 1

Purification and Some Catalytic Properties of Glucose-6-Phosphate Dehydrogenase Isoforms from Barley Leaves

Similar Documents

Glucose-6-phosphate dehydrogenase in barley roots: kinetic properties and localisation of the isoforms

Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties

Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties

Purification of Glucose 6-Phosphate Dehydrogenase From Goose Erythrocytes and Kinetic Properties

PURIFICATION AND PROPERTIES OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM CORIANDER (CORIANDRUM SATIVUM) LEAVES

Purification and Characteristics of Sorbitol-6-phosphate Dehydrogenase from Loquat Leaves 1

Glucose-6-phosphate Dehydrogenase from Agaricus bispovus : Purification and Properties

Purification and Properties of Microsomal Glucose 6-Phosphate Dehydrogenase ( Hexose 6-Phosphate Dehydrogenase ) of Rat Liver

Glucose 6-Phosphate Dehydrogenase Isozymes of Maize Leaves 1

Purification and Some Catalytic Properties of Glucose-6-Phosphate Dehydrogenase Isoforms from Barley Leaves