NDLI: Influence of in vitro growth conditions on the photosynthesis and survival of Castanea sativa plantlets during ex vitro transfer

Content Provider	Springer Nature Link
Author	Sáez, Patricia L. Bravo, León A. Latsague, Mirtha I. Toneatti, Marcelo J. Coopman, Rafael E. Álvarez, Carolina E. Sánchez Olate, Manuel Ríos, Darcy G.
Copyright Year	2014
Abstract	Adequate in vitro micro-environments are crucial to induce life compatible leaf development. Key morphological and physiological traits are needed to allow ex vitro survival. We study, how in vitro light and ventilation affect physiological performance and survival of ex vitro Castanea sativa plantlets. In vitro treatments consisted of two irradiances of 50 and 150 µmol m−2 s−1 in ventilated vessels (VL50 and VL150, respectively), compared to traditional cultures at 50 µmol m−2 s−1 in non-ventilated vessels (NVL50). After the exposure to each condition a photoinhibitory treatment (PhT) was also applied to study whether the above in vitro conditions exerted photoprotection and facilitated the recovery of C. sativa during sudden ex vitro transfer. During rooting, a decrease in net photosynthesis (Psat), electron transport rate (ETRII) and maximal efficiency of PSII (F v /F m ) were observed. Transpiration rates (E) decreased, concomitantly with a rise in water use efficiency (WUE), mainly in microplants originating from ventilated treatments (V). Throughout this stage, the PhT was lethal for all in vitro treatments. During acclimation, the number and leaf size increased principally in plantlets originating from V treatments. These microplants were also able to recover their ETR and F v /F m . Initially, the PhT produced a drastic drop in Fv/Fm of plantlets in all treatments however they did show a tendency to recover. Transferring plantlets to the greenhouse produced a decrease in the Psat in all treatments; however, over time Psat increased reaching values of 3.2 and 5.3 μmol CO2 m−2 s−1 in microplants originating from VL50 and VL150, respectively. Transpiration rate were similar in all treatments and remained at levels of about 0.9 mmol H2O m−2 s−1; thus, WUE increased significantly, reaching values of almost 3.8 µmol CO2/mmol H2O in microplants originating from VL150. After the PhT, all of the plantlet’s recovery capacity increased concomitantly with their dynamic heat dissipation and their de-epoxidation capacity. Our results suggest that managing in vitro conditions can improve plantlets photosynthetic performance in early stages after ex vitro transfer, playing a key role in the ameliorating the transfer stress.
Starting Page	625
Ending Page	639
Page Count	15
File Format	PDF
ISSN	01676903
Journal	Plant Growth Regulation
Volume Number	75
Issue Number	3
e-ISSN	15735087
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-09-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Acclimation Ex vitro transfer Fluorescence Photosynthesis Photoinhibition Plant Sciences Plant Anatomy/Development Plant Physiology Agriculture
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Agronomy and Crop Science

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