NDLI: Hydrophobic Barrier in Liposomes Studied by FT-ESEEM

Content Provider	Springer Nature Link
Author	ethig Laslo, V. Cevc, P. Arcon, D. Šentjurc, M.
Copyright Year	2004
Abstract	An improvement of the electron spin echo envelope modulation technique (ESEEM) for studying the hydrophobic barrier in lipid membranes was proposed. Water penetration depth into the lipid bilayer composed of egg-phosphatidylcholine and dicetylphosphate was studied. For this purpose the stearic acid spin probes with nitroxide moiety at different positions on the acyl chain were dissolved in the bilayer of liposomes prepared in buffer solution with D$_{2}$O. The ESEEM method is based on the deuterium effect to the $^{14}$N-hyperfine splitting constant of the spin probe. For the analysis of the spectra we suggested the frequency domain approach (Fourier transform-ESEEM) instead of the time domain. In order to test this approach, the influence of cholesterol and octanol on the water penetration depth into liposomes was measured. FT-ESEEM appeared to be a more sensitive method for the quantitative measurements of the water penetration depth than the time domain. This approach can become one of the most effective methods for studying the influence of surfactants and other biologically active molecules on water penetration depth into membranes.
Starting Page	237
Ending Page	242
Page Count	6
File Format	PDF
ISSN	01696149
Journal	Origins of Life and Evolution of Biospheres
Volume Number	34
Issue Number	1-2
e-ISSN	15730875
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Organic Chemistry Geochemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Ecology, Evolution, Behavior and Systematics Space and Planetary Science

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