NDLI: Dequalinium-Induced Cell Death of Yeast Expressing α-Synuclein-GFP Fusion Protein

Content Provider	Springer Nature Link
Author	Lee, In Hwan Kim, Hui Young Kim, Myunghye Hahn, Ji Sook Paik, Seung R.
Copyright Year	2008
Abstract	Intracellular toxic effects of the dequalinium-induced protofibrils of α-synuclein have been investigated with the yeast system expressing α-synuclein-GFP fusion protein in single copy, which appears in the green halo around the plasma membrane. Intracellular responses of the green fluorescent protein were analyzed as the cells were treated with dequalinium (DQ) and lactacystin. Yeast cells expressing α-synuclein-GFP were susceptible to both compounds in α-synuclein-dependent manner. Upon DQ treatment, the green halo became smeared throughout the cytoplasm while lactacystin induced a few discrete green dots, reflecting intracellular formation of the protofibrils and the protein inclusions, respectively. The DQ-treated yeast cells were intensely stained with the nucleic acid stains of cell-permeable Hoechst 33342 and cell-impermeable propidium imidione, indicating that nucleus has been disrupted in addition to plasma membrane destabilization. Those DQ-treated yeast cells, however, still contained active mitochondria identified with MitoTracker Red. Therefore, the DQ-induced protofibrillar state of α-synuclein-GFP has been suggested to cause the nuclear damage either independently or in combination with the membrane destabilization without affecting mitochondria.
Starting Page	1393
Ending Page	1400
Page Count	8
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	33
Issue Number	7
e-ISSN	15736903
Language	English
Publisher	Springer US
Publisher Date	2008-03-07
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dequalinium α-Synuclein Yeast Neurodegenerative disorder Cell death Lactacystin Nuclear malfunction Mitochondria dysfunction Parkinson’s disease Neurology Biochemistry Neurosciences
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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