NDLI: Quantification of Superoxide Radical in the Brain of Rats with Experimentally Induced Obstructive Jaundice

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Quantification of Superoxide Radical in the Brain of Rats with Experimentally Induced Obstructive Jaundice

Content Provider	Springer Nature Link
Author	Konstantiu, Dimitris Mavrakis, Adamantios Grintzalis, Konstantis Papapostolou, Ioannis Assimakopoulos, Stelios F. Chroni, Elisabeth Georgiou, Christos
Copyright Year	2007
Abstract	The study aimed to directly measure in vivo superoxide radical (O 2 − ) a direct indicator of oxidative stress, in the brain of rats with experimentally induced obstructive jaundice by employing a new quantitative ultrasensitive fluorescent assay requiring minimum sample. O 2 − anion is specific for dihydroethidine (DHE) and upon reaction gives a characteristic product, namely 2-OH-ethidium. Ten male rats underwent laparotomy and were divided into two groups: I, sham operated and II bile duct ligation. Ten days later, following injection with DHE (a O 2 − trap), all animals were killed and samples from cerebral cortex, midbrain and cerebellum were removed for analysis. It was shown that compared to group I, in group II the O 2 − was increased by 67% in the cerebral cortex and by 37% in the midbrain as a consequence of experimental obstructive jaundice, while its levels were unaffected in the cerebellum. The data in this experimental obstructive jaundice model imply a region-specific increase of O 2 − formation rate, being higher in cerebral cortex, less so in the midbrain and not at all in cerebellum.
Starting Page	1101
Ending Page	1105
Page Count	5
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	33
Issue Number	6
e-ISSN	15736903
Language	English
Publisher	Springer US
Publisher Date	2007-12-20
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Brain oxidative stress Obstructive jaundice Experimental hepatic encephalopathy Superoxide radical Dihydroethidine Reactive oxygen species Free radicals Neurology Biochemistry Neurosciences
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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