NDLI: Cytotoxicity evaluation of nanoclays in human epithelial cell line A549 using high content screening and real-time impedance analysis

Content Provider	Springer Nature Link
Author	Verma, Navin K. Moore, Edward Blau, Werner Volkov, Yuri Ramesh Babu, P.
Copyright Year	2012
Abstract	Continuously expanding use of products containing nanoclays for wide range of applications have raised public concerns about health and safety. Although the products containing nanoclays may not be toxic, it is possible that nanomaterials may come in contact with humans during handling, manufacture, or disposal, and cause adverse health impact. This necessitates biocompatibility evaluation of the commonly used nanoclays. Here, we investigated the cytotoxic effects of platelet (Bentone MA, ME-100, Cloisite Na$^{+}$, Nanomer PGV, and Delite LVF) and tubular (Halloysite, and Halloysite MP1) type nanoclays on cultured human lung epithelial cells A549. For the first time with this aim, we employed a cell-based automated high content screening in combination with real-time impedance sensing. We demonstrate varying degree of dose- and time-dependent cytotoxic effects of both nanoclay types. Overall, platelet structured nanoclays were more cytotoxic than tubular type. A low but significant level of cytotoxicity was observed at 25 μg/mL of the platelet-type nanoclays. A549 cells exposed to high concentration (250 μg/mL) of tubular structured nanoclays showed inhibited cell growth. Confocal microscopy indicated intracellular accumulation of nanoclays with perinuclear localization. Results indicate a potential hazard of nanoclay-containing products at significantly higher concentrations, which warrant their further biohazard assessment on the actual exposure in humans.
Starting Page	1
Ending Page	11
Page Count	11
File Format	PDF
ISSN	13880764
Journal	Journal of Nanoparticle Research
Volume Number	14
Issue Number	9
e-ISSN	1572896X
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-08-28
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nanoclay Cytotoxicity High content screening Impedance sensing Physical Chemistry Inorganic Chemistry Optics, Optoelectronics, Plasmonics and Optical Devices Characterization and Evaluation of Materials Nanotechnology
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Atomic and Molecular Physics, and Optics Condensed Matter Physics Bioengineering Modeling and Simulation

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Effects of serum on cytotoxicity of nano- and micro-sized ZnO particles

Mechanisms of nanoclay-enhanced plastic foaming processes: effects of nanoclay intercalation and exfoliation

Silica nanoparticles and biological dispersants: genotoxic effects on A549 lung epithelial cells

Multidisciplinary cognitive content of nanoscience and nanotechnology

The acute cytotoxicity of bismuth ferrite nanoparticles on PC12 cells

Nano-fillers to tune Young’s modulus of silicone matrix

Cyto- and genotoxicity assessment of Gold nanoparticles obtained by laser ablation in A549 lung adenocarcinoma cells

Cytotoxicity of semiconductor nanoparticles in A549 cells is attributable to their intrinsic oxidant activity

Cytotoxicity and drug release behavior of PNIPAM grafted on silica-coated iron oxide nanoparticles

Cytotoxicity evaluation of nanoclays in human epithelial cell line A549 using high content screening and real-time impedance analysis

Similar Documents

Effects of serum on cytotoxicity of nano- and micro-sized ZnO particles

Mechanisms of nanoclay-enhanced plastic foaming processes: effects of nanoclay intercalation and exfoliation

Silica nanoparticles and biological dispersants: genotoxic effects on A549 lung epithelial cells

Multidisciplinary cognitive content of nanoscience and nanotechnology

The acute cytotoxicity of bismuth ferrite nanoparticles on PC12 cells

Nano-fillers to tune Young’s modulus of silicone matrix

Cyto- and genotoxicity assessment of Gold nanoparticles obtained by laser ablation in A549 lung adenocarcinoma cells

Cytotoxicity of semiconductor nanoparticles in A549 cells is attributable to their intrinsic oxidant activity

Cytotoxicity and drug release behavior of PNIPAM grafted on silica-coated iron oxide nanoparticles

Cytotoxicity evaluation of nanoclays in human epithelial cell line A549 using high content screening and real-time impedance analysis