NDLI: Development of a new sensitive immunostrip assay based on mesoporous silica and colloidal Au nanoparticles

Content Provider	Springer Nature Link
Author	Omidfar, Kobra Khorsand, Behsh Larijani, Bagher
Copyright Year	2011
Abstract	A new competitive immunostrip assay was developed to detect human serum albumin (HSA) in urine sample with use of conjugated monoclonal antibody gold nanoparticles (mAb–AuNPs) and mobile crystalline material (MCM)-41–HSA bioconjugate. To prepare the immunostrip, the colloidal AuNPs with an average particle diameter of 20 nm, was synthesized, labeled with antibody and applied on the conjugate pad as the detection reagent. Then, HSA was attached to the MCM-41 mesoporous nanoparticles and immobilized to a nitrocellulose membrane as the test line. In the optimized investigational conditions, the immunostrip could detect HSA in a high linear range (from 1 to 200 μg/ml) and low detection limit (ng/ml). The reliability of the testing procedure was examined by performing the immunostrip test with 30 urine samples and comparing the results with those obtained via immunoturbidimetry. The immunostrip was adequately sensitive and accurate for a rapid screening of HSA in the urine. This new strategy for competitive immunostrip design can be used and developed for other antigen based immunostrip assay.
Starting Page	1253
Ending Page	1259
Page Count	7
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	39
Issue Number	2
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-05-21
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immunostrip assay Gold nanoparticles Mobile crystalline material silica nanoparticles Human serum albumin Microalbuminuria Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Interaction of ergosterol with bovine serum albumin and human serum albumin by spectroscopic analysis

Development of an enhanced chemiluminescence immunoassay (CLIA) for detecting urinary albumin

The specific binding of chlorogenic acid to human serum albumin

Study on the interaction of a copper(II) complex containing the artificial sweetener aspartame with human serum albumin

The binding characteristics of the interaction between 3-(2-cyanoethyl) cytosine and human serum albumin

Interaction of human serum albumin with 10-hydroxycamptothecin: spectroscopic and molecular modeling studies

Binding of berberine to bovine serum albumin: spectroscopic approach

Binding properties of food colorant allura red with human serum albumin in vitro

Interaction of artemisinin and its derivatives with human serum albumin studied using spectroscopies and molecular modeling methods

Development of a new sensitive immunostrip assay based on mesoporous silica and colloidal Au nanoparticles

Similar Documents

Interaction of ergosterol with bovine serum albumin and human serum albumin by spectroscopic analysis

Development of an enhanced chemiluminescence immunoassay (CLIA) for detecting urinary albumin

The specific binding of chlorogenic acid to human serum albumin

Study on the interaction of a copper(II) complex containing the artificial sweetener aspartame with human serum albumin

The binding characteristics of the interaction between 3-(2-cyanoethyl) cytosine and human serum albumin

Interaction of human serum albumin with 10-hydroxycamptothecin: spectroscopic and molecular modeling studies

Binding of berberine to bovine serum albumin: spectroscopic approach

Binding properties of food colorant allura red with human serum albumin in vitro

Interaction of artemisinin and its derivatives with human serum albumin studied using spectroscopies and molecular modeling methods

Development of a new sensitive immunostrip assay based on mesoporous silica and colloidal Au nanoparticles