NDLI: Anti-apoptotic and antioxidant effect of leptin on CCl$

Content Provider	Springer Nature Link
Author	Şerbetçi, Kerim Uysal, Onur Erkasap, Nilüfer Köken, Tülay Baydemir, Canan Erkasap, Serdar
Copyright Year	2011
Abstract	The aim of this study is to investigate the effect of leptin in rats on carbon tetrachloride (CCl$_{4}$) induced acute liver damage using immunohistochemical methods for apoptosis and biochemical parameters. In this experimental study, 18 Spraque-Dawley rats were divided into three groups viz; control, CCl$_{4}$ and CCl$_{4}$+leptin treatment. 0.8 ml/kg olive oil was administered intraperitoneally (i.p.) to the control group and 0.8 ml/kg CCl$_{4}$ (1:1 dissolved in olive oil) was administered i.p. to the CCl$_{4}$ and CCl$_{4}$+leptin treatment groups, respectively. After 6 h of administrating CCl$_{4}$, CCl$_{4}$+leptin treatment group was given i.p. leptin (10 μg/kg). Twenty-four hours after administrating CCl$_{4}$ all of the groups were euthanized. Biochemical assessments were performed using serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), plasma tumor necrosis factor alpha (TNF-α) levels and tissue malondialdehyde (MDA), and TNF-α levels. Histological assessments were then performed using Hematoxylin&Eosin (H&E) staining in light microscope and apoptosis assessment using Terminal Transferase dUTP Nick End Labeling (TUNEL)-staining. Serum AST, ALT, ALP and plasma TNF-α levels, tissue MDA and TNF-α levels had all increased in CCl$_{4}$ group, but were found to be significantly decreased in CCl$_{4}$+leptin treatment group. Moreover, TUNEL-positive cell counts in liver had significantly increased in CCl$_{4}$ group, but decreased in CCl$_{4}$+leptin treatment group (P < 0.05). The results of our study the biochemical, histological and TUNEL-staining showed that leptin has treatment effects on liver CCl$_{4}$ induced injury. It plays a role as a potent free radical scavenger, a powerful antioxidant and it also has anti-apoptotic effects.
Starting Page	1173
Ending Page	1180
Page Count	8
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	39
Issue Number	2
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-05-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Carbon tetrachloride Rat Liver Leptin Apoptosis Antioxidant Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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