NDLI: Sodium arsenite mediated immuno-disruption through alteration of transcription profile of cytokines in chicken splenocytes under in vitro system

Content Provider	Springer Nature Link
Author	Das, Subhashree Pan, Diganta Bera, Asit Kumar Rana, Tanmoy Bhattacharya, Debasis Bandyapadyay, Subhasis De, Sumanta Sreevatsava, V. Bhattacharya, Somnath Das, Subrata Kumar Bandyopadhayay, Sandip
Copyright Year	2010
Abstract	Arsenic is a ubiquitously found metalloid that commonly contaminates drinking water and agricultural food. To understand the ecotoxicological effects of arsenic in environment, it is essential to ameliorate the deleterious effects on human and animal health, particularly on the immune response. We investigated the effects of inorganic arsenic (iAs) on the immune response of chicken splenocytes. Both 1 and 10 mM concentrations of sodium arsenite treatment significantly reduced (P < 0.001) splenocyte proliferation and phagocytic activity compared to concanavalin A (ConA) stimulated cells at 24, 48 and 72 h of incubation. Nitrous oxide (NO) production was significantly higher (P < 0.001) at 24 h and subsequently declined in the higher dose group, while there was a gradual decline from 24 to 72 h in the lower dose group. Comparison of two different concentration of arsenic treatment also revealed time dependent differences. Relative quantification of expression of IFNγ and IL2 revealed that both genes were significantly down regulated (P < 0.001) at both concentrations at each time point. iNOS gene was rapidly down regulated in splenocytes at 24 h at the high doses of As treated splenocyte, a gradual decreasing trend at low doses. Down regulation of IL-2 gene expression in response to As was further evidenced by a significant reduction (P < 0.001) in the release of IL-2 into the splenocyte culture medium. We suggest that arsenic, a potent immunotoxic agent, modulates non-specific immune responses and alters the expression of cytokines in a dose and time dependent manner.
Starting Page	171
Ending Page	176
Page Count	6
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	38
Issue Number	1
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-03-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sodium arsenite IL-2 iNOS INF-γ Chicken splenocyte Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Use of zinc chloride as alternative stimulant for in vitro study of nitric oxide production pathway in avian splenocyte culture

Stress inducible heat shock protein 70: a potent molecular and toxicological signature in arsenic exposed broiler chickens

Protective effect of curcumin against arsenic-induced apoptosis in murine splenocytes in vitro

Adenovirus-mediated IL-24 confers radiosensitization to human lung adenocarcinoma in vitro and in vivo

Response to mitogens of chicken splenocytes determined by three methods of measurement

Interferon gamma and Interleukin 10 polymorphisms in Brazilian patients with systemic lupus erythematosus

Innate Activation of IFN-γ—iNOS Axis During Infection With Salmonella Represses the Ability of T Cells to Produce IL-2

Protective effect of myokine IL-15 against H$_{2}$O$_{2}$-mediated oxidative stress in skeletal muscle cells

Characterization of the expression profile of CRBP1 and CRBP3 gene in chicken

Sodium arsenite mediated immuno-disruption through alteration of transcription profile of cytokines in chicken splenocytes under in vitro system

Similar Documents

Use of zinc chloride as alternative stimulant for in vitro study of nitric oxide production pathway in avian splenocyte culture

Stress inducible heat shock protein 70: a potent molecular and toxicological signature in arsenic exposed broiler chickens

Protective effect of curcumin against arsenic-induced apoptosis in murine splenocytes in vitro

Adenovirus-mediated IL-24 confers radiosensitization to human lung adenocarcinoma in vitro and in vivo

Response to mitogens of chicken splenocytes determined by three methods of measurement

Interferon gamma and Interleukin 10 polymorphisms in Brazilian patients with systemic lupus erythematosus

Innate Activation of IFN-γ—iNOS Axis During Infection With Salmonella Represses the Ability of T Cells to Produce IL-2

Protective effect of myokine IL-15 against H$_{2}$O$_{2}$-mediated oxidative stress in skeletal muscle cells

Characterization of the expression profile of CRBP1 and CRBP3 gene in chicken

Sodium arsenite mediated immuno-disruption through alteration of transcription profile of cytokines in chicken splenocytes under in vitro system