NDLI: Isolation and characterization of a harvest-inducible gene hi11 and its promoter from alfalfa

Content Provider	Springer Nature Link
Author	Zhang, Jian Xiong, Ai Sheng Erickson, Larry R.
Copyright Year	2010
Abstract	The harvesting and storing of alfalfa is a routine practice in the agricultural industry worldwide. To investigate gene expression in harvested alfalfa, cDNA from non-harvested and harvested plants in the field was subjected to subtractive hybridization to identify, in particular, those genes that are induced by the harvesting treatment. One cDNA clone, named hi11, was isolated and analysed. The full length cDNA of the hi11 gene was cloned by RACE amplification. The hi11 gene, which has high homology to a putative protein of unknown function in Arabidopsis, was induced in alfalfa following harvesting, a 38°C heat shock and a wounding treatment. Northern blot analysis confirmed that the expression patterns of hi11 in alfalfa in response to harvesting, heat shock, and wounding. In addition, genomic walking was performed to isolate the 5′ flanking region of the hi11 gene. The promoter of the hi11 gene was fused to the GUS reporter gene and transferred to Medicago truncatula and tobacco. In all transgenic plants of M. truncatula and tobacco, GUS gene expression was observed in harvested tissue, especially in the transgenic tobacco plants, but not in the non-harvested control tissue.
Starting Page	23
Ending Page	29
Page Count	7
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	38
Issue Number	1
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-03-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Harvest-induced gene Subtractive hybridization RACE Genome walking hi11 Alfalfa Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Harvest-inducibility of the promoter of alfalfa S-adenosyl-l-methionine: trans-caffeoyl-CoA3-O-methyltransferase gene

Isolation and characterization of Histone1 gene and its promoter from tea plant (Camellia sinensis)

Screening of genes induced by salt stress from Alfalfa

Assay and characterization of an osmolarity inducible promoter newly isolated from Bacillus subtilis

Characterization of the porcine differentially expressed PDK4 gene and association with meat quality

Sequence and expression analysis of the gene encoding inducible cAMP early repressor in tilapia

Isolation and analysis of genes mainly expressed in adult mouse heart using subtractive hybridization cDNA library

Analysis of gene expression by ESTs from suppression subtractive hybridization library in Chenopodium album L. under salt stress

Isolation of high quality RNA and construction of a suppression subtractive hybridization library from ramie (Boehmeria nivea L. Gaud.)

Isolation and characterization of a harvest-inducible gene hi11 and its promoter from alfalfa

Similar Documents

Harvest-inducibility of the promoter of alfalfa S-adenosyl-l-methionine: trans-caffeoyl-CoA3-O-methyltransferase gene

Isolation and characterization of Histone1 gene and its promoter from tea plant (Camellia sinensis)

Screening of genes induced by salt stress from Alfalfa

Assay and characterization of an osmolarity inducible promoter newly isolated from Bacillus subtilis

Characterization of the porcine differentially expressed PDK4 gene and association with meat quality

Sequence and expression analysis of the gene encoding inducible cAMP early repressor in tilapia

Isolation and analysis of genes mainly expressed in adult mouse heart using subtractive hybridization cDNA library

Analysis of gene expression by ESTs from suppression subtractive hybridization library in Chenopodium album L. under salt stress

Isolation of high quality RNA and construction of a suppression subtractive hybridization library from ramie (Boehmeria nivea L. Gaud.)

Isolation and characterization of a harvest-inducible gene hi11 and its promoter from alfalfa