NDLI: Identification of genes differentially expressed in cauliflower associated with resistance to Xanthomonas campestris pv. campestris

Content Provider	Springer Nature Link
Author	Jiang, Hanmin Song, Wenqin Li, Ai Yang, Xiao Sun, Deling
Copyright Year	2010
Abstract	Black rot, caused by Xanthomonas campestris pv. campestris (Pammel) Dowson (Xcc), is one of the most damaging diseases of cauliflower and other crucifers. In order to investigate the molecular resistance mechanisms and to find the genes related to black rot resistance in cauliflower, a suppression subtractive hybridization (SSH) cDNA library was constructed using resistant line C712 and its susceptible near-isogenic line C731 as tester and driver, respectively. A total of 280 clones were obtained from the library by reverse northern blotting. Sequencing analysis and homology searching showed that these clones represent 202 unique sequences. The library included many defense/disease-resistant related genes, such as plant defensin gene PDF1.2, lipid transfer protein, thioredoxin h. Gene expression profiles of 12 genes corresponding to different functional categories were monitored by real-time RT-PCR. The results showed that the expression induction of these genes in the susceptible line C712 in response to Xcc was quicker and more intense, while in C731 the reaction was delayed and limited. Our results imply that these up-regulated genes might be involved in cauliflower responses against Xcc infection. Information obtained from this study could be used to understand the molecular mechanisms of disease response in cauliflower under Xcc stress.
Starting Page	621
Ending Page	629
Page Count	9
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	38
Issue Number	1
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-04-16
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Black rot Brassica oleracea var. botrytis L. Suppression subtractive hybridization Xanthomonas campestris pv. campestris (Pammel) Dowson Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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Investigations on black rot of cauliflower (Brassica oleracea var. botrytis L.) caused by Xanthomonas campestris pv. campestris (Pammel) Dowson

Management of black-rot in cauliflower caused by Xanthomonas campestris pv. campestris (Pammel) Dowson

Studies on Black Rot (Xanthomonas campestris pv. compestris (Pammel) Dowson) of Cabbage (Brassica Oleracea var. Capitata) Under South gujarat condition

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STUDIES ON GENE TAGGING FOR RESISTANCE TO Xanthomonas campestris IN CAULIFLOWER (Brassica oleracea var. botrytis) USING SSR MARKERS

Identification of genes differentially expressed in cauliflower associated with resistance to Xanthomonas campestris pv. campestris

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