NDLI: Seed-specific expression of a lysine rich protein sb401 gene significantly increases both lysine and total protein content in maize seeds

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Seed-specific expression of a lysine rich protein sb401 gene significantly increases both lysine and total protein content in maize seeds

Content Provider	Springer Nature Link
Author	Yu, Jingjuan Peng, Peng Zhang, Xiujun Zhao, Qian Zhy, Dengyun Sun, Xuehui Liu, Junqi Ao, Guangming
Copyright Year	2004
Abstract	The sb401 gene from potato (Solanum berthaultii) encoding a pollen-specific protein with high lysine content was successfully integrated into the genome of maize plants and its expression was correlated with increased levels of lysine and total protein content in maize seeds. A plasmid vector containing the sb401 gene under the control of a maize seed-specific expression storage protein promoter (P19z) was constructed and introduced into maize calli using microprojectile bombardment. The integration of the sb401 gene into the maize genome was confirmed by Southern blot analysis and its expression was confirmed by Western blot analysis. Quantification of lysine and protein content in R1 maize seeds showed that, compared to the non-transgenic maize control, the lysine content increased by 16.1% to 54.8%, and total protein content increased by 11.6% to 39.0%. There was no visible morphological change in vegetative parts and seeds of the transgenic maize plants. Lysine and protein analysis of the transgenic maize grains showed that the levels of lysine and total protein remained high for six continuous generations, indicating that the elevated lysine and total protein levels were heritable. These results indicate that the sb401 gene could be successfully employed in breeding programmes aimed at improving the nutritional value of maize.
Starting Page	1
Ending Page	7
Page Count	7
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	14
Issue Number	1
e-ISSN	15729788
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2004-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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