NDLI: Pigmented Maize Seed via Tissue-specific Expression of Anthocyanin Pathway Gene Transcription Factors

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Pigmented Maize Seed via Tissue-specific Expression of Anthocyanin Pathway Gene Transcription Factors

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Pigmented Maize Seed via Tissue-specific Expression of Anthocyanin Pathway Gene Transcription Factors

Content Provider	Springer Nature Link
Author	Shen, Liu Y. Petoli, Joseph F.
Copyright Year	2006
Abstract	A visual marker genetically linked to a trait of interest would enable the unequivocal identification of transgenic seed containing the trait and ensure that genetically modified material could be easily differentiated from non-transgenic grain. Genes from maize that regulate the anthocyanin biosynthetic pathway, and are therefore responsible for directing the pattern of purple pigmentation, can be used to generate transgenic plants with unique, easily recognizable phenotypes. In the present study, a seed-specific maize globulin promoter was used to drive the expression of two transcription factor genes (Bp and C1) regulating the anthocyanin biosynthetic pathway in embryo and aleurone tissues resulting in maize seed with a distinct purple pigmentation. The following report describes the construction of expression vectors containing these two genes and an herbicide resistance selectable marker gene, the generation and characterization of transgenic cultures, the induction of pigmentation in somatic embryos, the regeneration of transgenic plants with pigmented seed and the co-segregation of integrated DNA with seed pigmentation and herbicide resistance. The co-segregation of seed pigmentation and herbicide resistance confirms the feasibility of using the expression of these genes in a ‘molecular stack’ for visual transgene identification.
Starting Page	57
Ending Page	67
Page Count	11
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	18
Issue Number	1
e-ISSN	15729788
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-06-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Agrobacterium transformation Identity preservation Globulin promoter Seed pigmentation Visual marker Transgene inheritance Co-segregation Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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