NDLI: Over-expression of the MxIRT1 gene increases iron and zinc content in rice seeds

Content Provider	Springer Nature Link
Author	Tan, Song Han, Rui Li, Peng Yang, Guang Li, Shuang Zhang, Peng Wang, Wei Bing Zhao, Wei Zhong Yin, Li Ping
Copyright Year	2014
Abstract	Iron and zinc are essential in plant and human nutrition. Iron deficiency has been one of the causes of human mortality, especially in developing countries with high rice consumption. MxIRT1 is a ferrous transporter that has been screened from an iron-efficient genotype of the apple tree, Malus xiaojinensis Cheng et Jiang. In order to produce Fe-biofortified rice with MxIRT1 to solve the Fe-deficiency problem, plant expression vectors of pCAMBIA1302-MxIRT1:GFP and pCAMBIA1302-anti MxIRT1:GFP were constructed that led to successful production of transgenic rice. The transgenic plant phenotypes showed that the expression of endogenous OsIRT1 was suppressed by anti-MxIRT1 in antisense lines that acted as an opposing control, while sense lines had a higher tolerance under Zn- and Fe-deficient conditions. The iron and zinc concentration in T$_{3}$ seeds increased by three times in sense lines when compared to the wild type. To understand the MxIRT1 cadmium uptake, the MxIRT1 cadmium absorption trait was compared with AtIRT1 and OsIRT1 in transgenic rice protoplasts, and it was found that MxIRT1 had the lowest Cd uptake capacity. MxIRT1 transgenic tobacco-cultured bright yellow-2 (BY-2) cells and rice lines were subjected to different Fe conditions and the results from the non-invasive micro-test technique showed that iron was actively transported compared to cadmium as long as iron was readily available in the environment. This suggests that MxIRT1 is a good candidate gene for plant Fe and Zn biofortification.
Starting Page	109
Ending Page	122
Page Count	14
File Format	PDF
ISSN	09628819
Journal	Transgenic Research
Volume Number	24
Issue Number	1
e-ISSN	15739368
Language	English
Publisher	Springer International Publishing
Publisher Date	2014-08-07
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	IRT1 Iron Biofortification Rice seed Cadmium NMT Molecular Medicine Plant Genetics & Genomics Animal Genetics and Genomics Plant Sciences Transgenics
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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