NDLI: Mutation loci and intragenic selection marker of the granule-bound starch synthase gene in waxy maize

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Mutation loci and intragenic selection marker of the granule-bound starch synthase gene in waxy maize

Content Provider	Springer Nature Link
Author	Liu, Jian Rong, Tingzhao Li, Wanchen
Copyright Year	2007
Abstract	Four pairs of specific PCR primers have been designed on the basis of the sequence of the granule-bound starch synthase gene (GBSS; dominant non-waxy gene Wx) and used to amplify its homologous sequence from thirteen waxy and two non-waxy inbred lines. Results from electrophoresis indicated that the recessive waxy gene was wx, derived from the dominant non-waxy gene Wx by mutation at its 3′ end. The sequence of the mutated 3′ end was amplified by the TAIL-PCR technique. Sequence alignment showed that the mutation of the wx gene was caused by transposition of the aldehyde dehydrogenase gene rf2. Two pairs of specific primers were designed on the basis of the sequence difference between the dominant gene Wx and its mutated recessive allele wx and used as intragenic selection markers to identify individual plants of genotypes WxWx, Wxwx, and wxwx by PCR amplification from the segregating population of the F$_{2}$ generation crossed between waxy and non-waxy inbred lines. Iodine solution staining and starch component assay showed that all the 35 F$_{2}$ plants identified as genotype WxWx produced non-waxy kernels of the F$_{3}$ generation and that all 33 F$_{2}$ plants identified as genotype wxwx produced waxy kernels of the F$_{3}$ generation. This result can be used to improve the selection efficiency of waxy maize breeding and for selection of other single genes and major polygenes.
Starting Page	93
Ending Page	102
Page Count	10
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	20
Issue Number	2
e-ISSN	15729788
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2007-06-02
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Granule-bound starch synthase gene Intragenic selection marker Mutation locus TAIL-PCR Waxy maize Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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