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Identification and characterization of a novel Waxy allele from a Yunnan rice landrace

Content Provider	Springer Nature Link
Author	Liu, Linglong Ma, Xiaodong Liu, Shijia Zhu, Changlan Jiang, Ling Wang, Yihua Shen, Yi Ren, Yulong Dong, Hui Chen, Liangming Liu, Xi Zhao, Zhigang Zhai, Huqu Wan, Jianmin
Copyright Year	2009
Abstract	Low amylose content (AC) is a desirable trait for rice (Oryza sativa L.) cooking quality and is selected in soft rice breeding. To gain a better understanding of the molecular mechanism controlling AC formation, we screened 83 Yunnan rice landraces in China and identified a rice variety, Haopi, with low AC. Genetic analyses and transgenic experiments revealed that low AC in Haopi was controlled by a novel allele of the Wx locus, Wx $^{ hp }$, encoding a granule-bound starch synthase (GBSSI). Sequence comparisons of Wx $^{ hp }$ and Wx $^{ b }$ alleles (from Nipponbare) showed several nucleotide changes in the upstream regulatory regions (including the promoter, 5′-untranslated region, and first intron 5′ splicing junction site). Interestingly, these changes had no obvious effect on the expression level and splicing efficiency of Wx transcripts. In addition, an examination of the coding region revealed that the Wx $^{ hp }$ allele carries an A-to-G change at nucleotide position +497 from the start codon, resulting in an Asp$^{165}$/Gly$^{165}$ substitution. The amino acid substitution had no detectable effects on GBSSI activity in vitro; however, it notably reduced the binding of GBSSI to starch granules, resulting in a reduction of AC in rice seeds. Moreover, three other Yunnan landraces with low AC also carry a nucleotide substitution identical to Haopi at the +497 position of the Wx gene, suggesting common ancestry. Based on the single-nucleotide polymorphism, we have developed a new derived cleaved amplified polymorphic sequence marker for use in breeding practice to manipulate AC in rice endosperm.
Starting Page	609
Ending Page	626
Page Count	18
File Format	PDF
ISSN	01674412
Journal	Plant Molecular Biology
Volume Number	71
Issue Number	6
e-ISSN	15735028
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-09-17
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Rice (Oryza sativa L.) Waxy gene Granule-bound starch synthase (GBSSI) Amylose content dCAPS marker Plant Pathology Biochemistry Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Agronomy and Crop Science

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