NDLI: Improved Cassava Starch by Antisense Inhibition of Granule-bound Starch Synthase I

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Improved Cassava Starch by Antisense Inhibition of Granule-bound Starch Synthase I

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Molecular Breeding : Volume 16, Issue 1, August 2005

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Improved Cassava Starch by Antisense Inhibition of Granule-bound Starch Synthase I

Content Provider	Springer Nature Link
Author	Raemakers, Krit Schreuder, Marianne Suurs, Luc Furrer Verhorst, Heleen Vincken, Jean Paul Vetten, Nick Jacobsen, Evert Visser, Richard G. F.
Copyright Year	2005
Abstract	Cassava is a poor man's crop which is mainly grown as a subsistence crop in many developing countries. Its commercial use was first as animal feed (also known as tapioca), but has shifted since the late sixties to a source of native starch. The availability of native starches, which on the one hand do not require substantial chemical derivatisation and on the other hand have improved properties, would make cassava also for small farmers a potentially attractive cash crop. Since breeding is difficult in this polyploid, vegetatively propagated, crop a transgenic approach would be ideal to improve certain characteristics. We have created a cassava genotype producing amylose-free starch by genetic modification. The absence of amylose increased the clarity and stability of gels made with the transgenic starch, without requiring treatment with environment-unfriendly chemicals such as epoxides (propylene oxide, ethylene oxide) and acetic anhydride, which are normally used to improve stability. The amylose-free starch showed no changes in particle size distribution, chain length distribution or phosphorous content when compared to amylose-containing starch, but the granule melting temperature was increased by almost 2°C. Furthermore, the amylose-free cassava starch shows enhanced clarity and stability properties. These improved functionalities are desired in technical applications in paper and textile manufacturing, but also in the food industry for the production of sauces, dairy products and noodles.
Starting Page	163
Ending Page	172
Page Count	10
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	16
Issue Number	2
e-ISSN	15729788
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Amylose-free starch Cassava Genetic transformation Granule-bound starch synthase Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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Improved Cassava Starch by Antisense Inhibition of Granule-bound Starch Synthase I

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Improved Cassava Starch by Antisense Inhibition of Granule-bound Starch Synthase I

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