NDLI: Safety and mechanism of appetite suppression by a novel hydroxycitric acid extract (HCA-SX)

Content Provider	Springer Nature Link
Author	Ohia, Sunny E. Opere, Catherine A. LeDay, Angela M. Bagchi, Manashi Bagchi, Debasis Stohs, Sidney J.
Copyright Year	2002
Abstract	A growing body of evidence demonstrates the efficacy of Garcinia cambogia-derived natural (–)-hydroxycitric acid (HCA) in weight management by curbing appetite and inhibiting body fat biosynthesis. However, the exact mechanism of action of this novel phytopharmaceutical has yet to be fully understood. In a previous study, we showed that in the rat brain cortex a novel HCA extract (HCA-SX, Super CitriMax™) increases the release/availability of radiolabeled 5-hydroxytryptamine or serotonin ([$^{3}$H]-5-HT), a neurotransmitter implicated in the regulation of eating behavior and appetite control. The aim of the present study was 2-fold: (a) to determine the effect of HCA-SX on 5-HT uptake in rat brain cortex in vitro; and (b) to evaluate the safety of HCA-SX in vivo. Isolated rat brain cortex slices were incubated in oxygenated Krebs solution for 20 min and transferred to buffer solutions containing [$^{3}$H]-5-HT for different time intervals. In some experiments, tissues were exposed to HCA-SX (10 μM – 1 mM) and the serotonin receptor reuptake inhibitors (SRRI) fluoxetine (100 μM) plus clomipramine (10 μM). Uptake of [$^{3}$H]-5-HT was expressed as d.p.m./mg wet weight. A time-dependent uptake of [$^{3}$H]-5-HT occurred in cortical slices reaching a maximum at 60 min. HCA-SX, and fluoxetine plus clomipramine inhibited the time-dependent uptake of [$^{3}$H]-5-HT. At 90 min, HCA-SX (300 μM) caused a 20% decrease, whereas fluoxetine plus clomipramine inhibited [$^{3}$H]-5-HT uptake by 30%. In safety studies, acute oral toxicity, acute dermal toxicity, primary dermal irritation and primary eye irritation, were conducted in animals using various doses of HCA-SX. Results indicate that the LD$_{50}$ of HCA-SX is greater than 5000 mg/kg when administered once orally via gastric intubation to fasted male and female Albino rats. No gross toxicological findings were observed under the experimental conditions. Taken together, these in vivo toxicological studies demonstrate that HCA-SX is a safe, natural supplement under the conditions it was tested. Furthermore, HCA-SX can inhibit [$^{3}$H]-5-HT uptake (and also increase 5-HT availability) in isolated rat brain cortical slices in a manner similar to that of SRRIs, and thus may prove beneficial in controlling appetite, as well as treatment of depression, insomnia, migraine headaches and other serotonin-deficient conditions.
Starting Page	89
Ending Page	103
Page Count	15
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	238
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2002-01-01
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cardiology Medical Biochemistry Oncology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

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