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| Content Provider | Springer Nature Link |
|---|---|
| Author | Chikhirzhina, E. V. Polyanichko, A. M. Kostyleva, E. I. Vorobyev, V. I. |
| Copyright Year | 2011 |
| Abstract | Mechanisms of interaction of DNA with nonhistone chromosomal protein HMGB1 and linker histone H1 have been studied by means of circular dichroism and absorption spectroscopy. Both proteins are located in the internucleosomal regions of chromatin. It is demonstrated that the properties of DNA-protein complexes depend on the protein content and cannot be considered as a mere summing up of the effects of individual protein components. Interaction of the HMGB1 and H1 proteins is shown with DNA to be cooperative rather than competitive. Lysine-rich histone H1 facilitates the binding of HMGB1 to DNA by screening the negatively charged groups of the sugar-phosphate backbone of DNA and dicarboxylic amino acid residues in the C-terminal domain of HMGB1. The observed joint action of HMGB1 and H1 stimulates DNA condensation with the formation of anisotropic DNA-protein complexes with typical ψ-type CD spectra. Structural organization of the complexes depends not only on DNA-protein interactions but also on interaction between the HMGB1 and H1 protein molecules bound to DNA. Manganese ions significantly modify the mode of interactions between components in the triple DNA-HMGB1-H1 complex. The binding of Mn$^{2+}$ ions weakens DNA-protein interactions and strengthens protein-protein interactions, which promote DNA condensation and formation of large DNA-protein particles in solution. |
| Starting Page | 318 |
| Ending Page | 326 |
| Page Count | 9 |
| File Format | |
| ISSN | 00268933 |
| Journal | Molecular Biology |
| Volume Number | 45 |
| Issue Number | 2 |
| e-ISSN | 16083245 |
| Language | English |
| Publisher | SP MAIK Nauka/Interperiodica |
| Publisher Date | 2011-04-26 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | CD spectroscopy DNA DNA-protein interactions linker histone H1 manganese ions nonhistone protein HMGB1 Human Genetics Biochemistry Life Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Biophysics |
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