NDLI: The Effect of Ca$^{2+}$ Ions on DNA Compaction in the Complex with HMGB1 Nonhistone Chromosomal Protein

Content Provider	Springer Nature Link
Author	Polyanichko, A. M. Chikhirzhina, E. V. Andrushchenko, V. V. Kostyleva, E. I. Wieser, H. Vorob'ev, V. I.
Copyright Year	2004
Abstract	The analysis of absorption and circular dichroism spectra in UV and IR regions showed that Ca$^{2+}$ ions interact with the phosphate groups of DNA and the HMGB1 protein. Not only the negatively charged C-terminal part of the protein molecule, but also its DNA-binding domains participate in the interaction with metal ions. The latter leads to a change in the mode of protein–DNA interaction. The presence of Ca$^{2+}$ ions prevents the formation of ordered supramolecular structures specific for the HMGB1–DNA complexes but promotes intermolecular aggregation. The structure of DNA complexes with the HMGB1 protein lacking the C-terminal tail appeared to be the most sensitive to the presence of Ca$^{2+}$ ions. These data indicate that Ca$^{2+}$ ions play no structural role in the HMGB1–DNA complexes, and their presence is not necessary for DNA compaction in such systems.
Starting Page	590
Ending Page	600
Page Count	11
File Format	PDF
ISSN	00268933
Journal	Molecular Biology
Volume Number	38
Issue Number	4
e-ISSN	16083245
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2004-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Life Sciences Biochemistry
Content Type	Text
Resource Type	Article
Subject	Structural Biology Biophysics

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The Effect of Ca$^{2+}$ Ions on DNA Compaction in the Complex with HMGB1 Nonhistone Chromosomal Protein