NDLI: Protein Production and Crystallization at SECSG

Content Provider	Springer Nature Link
Author	Wang, Bi Cheng Adams, Michael W.W. Dailey, Harry DeLucas, Larry Luo, Ming Rose, John Bunzel, Robert Dailey, Tamara Habel, Jeff Horanyi, Peter Jenney, Francis E. Kataeva, Irina Lee, Han Seung Li, Songlin Li, Ting Lin, Dawei Liu, Zhi Jie Luan, Chi Hao Mayer, Michael Nagy, Lisa Newton, M. Gary Ng, Joseph Poole, Farris L. Shah, Ashit Shah, Claudia Sugar, Frank J. Xu, Hao
Copyright Year	2005
Abstract	Using a high degree of automation, the Southeast Collaboratory for Structural Genomics (SECSG) has developed high throughput pipelines for protein production, and crystallization using a two-tiered approach. Primary, or tier-1, protein production focuses on producing proteins for members of large Pfam families that lack a representative structure in the Protein Data Bank. Target genomes are Pyrococcus furiosus and Caenorhabditis elegans. Selected human proteins are also under study. Tier-2 protein production, or target rescue, focuses on those tier-1 proteins, which either fail to crystallize or give poorly diffracting crystals. This two tier approach is more efficient since it allows the primary protein production groups to focus on the production of new targets while the tier-2 efforts focus on providing additional sample for further studies and modified protein for structure determination. Both efforts feed the SECSG high throughput crystallization pipeline, which is capable of screening over 40 proteins per week. Details of the various pipelines in use by the SECSG for protein production and crystallization, as well as some examples of target rescue are described.
Starting Page	233
Ending Page	243
Page Count	11
File Format	PDF
ISSN	1345711X
Journal	Journal of Structural and Functional Genomics
Volume Number	6
Issue Number	2-3
e-ISSN	15700267
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	crystallization protein production SECSG Southeast Collaboratory structural genomics target salvaging Human Genetics Bioinformatics Microbial Genetics and Genomics Plant Genetics & Genomics Animal Genetics and Genomics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Genetics Structural Biology Biochemistry

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