NDLI: Structural Genomics of Eukaryotic Targets at a Laboratory Scale

Content Provider	Springer Nature Link
Author	Busso, Didier Poussin Courmontagne, Pierre Rosé, David Ripp, Raymond Litt, Alain Thierry, Jean Claude Moras, Di
Copyright Year	2005
Abstract	Structural genomics programs are distributed worldwide and funded by large institutions such as the NIH in United-States, the RIKEN in Japan or the European Commission through the SPINE network in Europe. Such initiatives, essentially managed by large consortia, led to technology and method developments at the different steps required to produce biological samples compatible with structural studies. Besides specific applications, method developments resulted mainly upon miniaturization and parallelization. The challenge that academic laboratories faces to pursue structural genomics programs is to produce, at a higher rate, protein samples. The Structural Biology and Genomics Department (IGBMC – Illkirch – France) is implicated in a structural genomics program of high eukaryotes whose goal is solving crystal structures of proteins and their complexes (including large complexes) related to human health and biotechnology. To achieve such a challenging goal, the Department has established a medium-throughput pipeline for producing protein samples suitable for structural biology studies. Here, we describe the setting up of our initiative from cloning to crystallization and we demonstrate that structural genomics may be manageable by academic laboratories by strategic investments in robotic and by adapting classical bench protocols and new developments, in particular in the field of protein expression, to parallelization.
Starting Page	81
Ending Page	88
Page Count	8
File Format	PDF
ISSN	1345711X
Journal	Journal of Structural and Functional Genomics
Volume Number	6
Issue Number	2-3
e-ISSN	15700267
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	cloning crystallization expression Gateway TM high throughput screening recombinant protein structural genomics Human Genetics Bioinformatics Microbial Genetics and Genomics Plant Genetics & Genomics Animal Genetics and Genomics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Genetics Structural Biology Biochemistry

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