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| Content Provider | Springer Nature Link |
|---|---|
| Author | Kalnina, Inta Bruvere, Ruta Zvagule, Tija Gabruseva, Natalija Klimkane, Laura Kirilova, Elena Meirovics, Imants Kizane, Gunta |
| Copyright Year | 2009 |
| Abstract | The fluorescent probe ABM (3-aminobenzanthrone derivative) one of the fluorescent probes synthesized in Riga Technical University proved to be an excellent, independent model for studying cell membranes. In our work we have investigated the possibility of using the fluorescent probe ABM for detection of immune state in patients with different pathologies. There is a strong correlation among all studied ABM spectral parameters, immunological characteristics, clinical and laboratory investigations of the all observed patients groups. The obtained results suggest that ABM spectral parameters in cell suspension reflect the alterations of the cellular mechanisms of immunity. Therefore fluorescent method could be used as preliminary screening test in immune diagnostics instead of more expensive, time consuming methods (subset detection, radioisotope method etc.) used as routine in clinics. Spectral parameters of ABM reflect a wide range of interrelated (interdependent) characteristics of cells (physico-chemical state and microviscosity of membrane, proliferating and lipid metabolic activity of cells, distribution of cells among subsets). The observed change of the studied parameters reflects alterations of the cellular mechanisms of immunity which is a main focus for its application as preliminary screening test in immune diagnostics. The fluorescence based method is sensitive, less expensive and time consuming, technically simple and convenient. |
| Starting Page | 9 |
| Ending Page | 17 |
| Page Count | 9 |
| File Format | |
| ISSN | 10530509 |
| Journal | Journal of Fluorescence |
| Volume Number | 20 |
| Issue Number | 1 |
| e-ISSN | 15734994 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2009-08-01 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Fluorescent probe Peripheral blood mononuclear cells Immune state Different pathologies Radiation effects Analytical Chemistry Biochemistry Biotechnology Biophysics and Biological Physics Biomedicine general |
| Content Type | Text |
| Resource Type | Article |
| Subject | Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology |
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