NDLI: Detection of Bifidobacterium Species-specific 16S rDNA Based on QD FRET Bioprobe

Content Provider	Springer Nature Link
Author	Jiang, JunFeng Peng, ZhiHui Deng, Le Li, Guang Chen, LinLi
Copyright Year	2009
Abstract	Fluorescence resonance energy transfer (FRET) that consists of quantum dot as donors and organic fluorophore dyes as acceptors has been a very important method to detect biomolecules such as nucleic acids. In this work, we established a new FRET detection system of Bifidobacterium species-specific 16S rDNA using QD—ROX FRET bioprobe, in which 525 nm QD-DNA conjugation consisted of the carboxyl-modified QD and the amino-modified DNA in the presence of EDC. Both ROX-DNA and the conjugation above could hybridize with the target DNA after forming the QD—ROX bioprobe. When the hybridization made the distance between the QD and ROX to meet FRET effect needed, 525 nm QD fluorescence intensity decreased and ROX fluorescence intensity increased. In the control, there was no notable change of fluorescence intensities without target DNA. It is very clear that the change of the QD and ROX fluorescence intensities provide the good base and guaranty for this rapid and simple detection system.
Starting Page	365
Ending Page	369
Page Count	5
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	20
Issue Number	1
e-ISSN	15734994
Language	English
Publisher	Springer US
Publisher Date	2009-09-26
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Quantum dots Sequence alignment Bioprobe FRET 16S rDNA detection Analytical Chemistry Biochemistry Biotechnology Biophysics and Biological Physics Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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