NDLI: The role of encapsulation by β-cyclodextrin in the interaction of raloxifene with macromolecular targets: a study by spectroscopy and molecular modeling

Content Provider	Springer Nature Link
Author	Sameena, Y. Sudha, N. Chandrasekaran, S. Ech, Israel V. M. V.
Copyright Year	2014
Abstract	We report the binding of the drug raloxifene with Calf thymus DNA (ctDNA) and bovine serum albumin (BSA) in the presence and absence of β-cyclodextrin (β-CD) and explain the influence of β-cyclodextrin on the binding of the drug to macromolecules. UV-Vis absorption, fluorescence, proton nuclear magnetic resonance and two-dimensional rotating-frame nuclear overhauser effect spectroscopic techniques are used to study the stoichiometry and the binding strength of the complexes. Molecular modeling is used in combination with other techniques to propose the structure of the inclusion complex and the interaction with ctDNA. The Stern–Volmer quenching constants of the interaction of raloxifene with ctDNA in aqueous and in β-CD solution are compared. The competition for binding of ctDNA with raloxifene and Methylene Blue is studied. The apparent binding constant and the number of binding sites for the binding of raloxifene with BSA in aqueous solution are significantly different from those in the presence of β-CD. The influence of β-CD on the binding of the small molecules with biological macromolecules is discussed. We infer that the binding strengths between raloxifene and macromolecules, viz., ctDNA and BSA are influenced by the β-CD encapsulation. These results may suggest new ways to tune the drug binding to biomacromolecules by encapsulating specific moieties of drugs.
Starting Page	347
Ending Page	367
Page Count	21
File Format	PDF
ISSN	00920606
Journal	Journal of Biological Physics
Volume Number	40
Issue Number	4
e-ISSN	15730689
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-07-30
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Raloxifene β-Cyclodextrin Calf thymus DNA Fluorescence spectroscopy Biophysics and Biological Physics Biochemistry Statistical Physics, Dynamical Systems and Complexity Neurosciences Soft and Granular Matter, Complex Fluids and Microfluidics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Atomic and Molecular Physics, and Optics Molecular Biology Biophysics

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