NDLI: NYGGF4 (PID1) effects on insulin resistance are reversed by metformin in 3T3-L1 adipocytes

Content Provider	Springer Nature Link
Author	Qiu, Jie Wang, Yu mei Shi, Chun mei Yue, Hong ni Qin, Zhen Ying Zhu, Guan zhong Cao, Xin guo Ji, Chen bo Cui, Yan Guo, Xi rong
Copyright Year	2012
Abstract	NYGGF4 (also called PID1) is a recently discovered gene that is involved in obesity-related insulin resistance (IR). We aimed in the present study to further elucidate the effects of NYGGF4 on IR and the underlying mechanisms through using metformin treatment in 3T3-L1 adipocytes. Our data showed that the metformin pretreatment strikingly enhanced insulin-stimulated glucose uptake through increasing GLUT4 translocation to the PM in NYGGF4 overexpression adipocytes. NYGGF4 overexpression resulted in significant inhibition of tyrosine phosphorylation of IRS-1 and serine phosphorylation of Akt, whereas incubation with metformin strongly activated IRS-1 and Akt phosphorylation in NYGGF4 overexpression adipocytes. The reactive oxygen species (ROS) levels in NYGGF4 overexpression adipocytes were strikingly enhanced, which could be decreased by the metformin pretreatment. Our data also showed that metformin increased the expressions of PGC1-α, NRF-1, and TFAM, which were reduced in the NYGGF4 overexpression adipocytes. These results suggest that NYGGF4 plays a role in IR and its effects on IR could be reversed by metformin through activating IRS-1/PI3K/Akt and AMPK-PGC1-α pathways.
Starting Page	665
Ending Page	671
Page Count	7
File Format	PDF
ISSN	0145479X
Journal	Journal of Bioenergetics and Biomembranes
Volume Number	44
Issue Number	6
e-ISSN	15736881
Language	English
Publisher	Springer US
Publisher Date	2012-09-12
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Obesity Insulin resistance NYGGF4 Metformin Adipocytes Bioorganic Chemistry Organic Chemistry Animal Anatomy / Morphology / Histology Biochemistry Animal Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Physiology

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