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  1. Journal of Materials Science: Materials in Medicine
  2. Journal of Materials Science: Materials in Medicine : Volume 23
  3. Journal of Materials Science: Materials in Medicine : Volume 23, Issue 11, November 2012
  4. Novel pHEMA-gelatin SPHs as bone scaffolds in dynamic cultures
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Journal of Materials Science: Materials in Medicine : Volume 28
Journal of Materials Science: Materials in Medicine : Volume 27
Journal of Materials Science: Materials in Medicine : Volume 26
Journal of Materials Science: Materials in Medicine : Volume 25
Journal of Materials Science: Materials in Medicine : Volume 24
Journal of Materials Science: Materials in Medicine : Volume 23
Journal of Materials Science: Materials in Medicine : Volume 23, Issue 12, December 2012
Journal of Materials Science: Materials in Medicine : Volume 23, Issue 11, November 2012
Biomimetic apatite-based biomaterials: on the critical impact of synthesis and post-synthesis parameters
Stiff gelatin hydrogels can be photo-chemically synthesized from low viscous gelatin solutions using molecularly functionalized gelatin with a high degree of methacrylation
Preparation and characterization of mesoporous bioactive glass/polycaprolactone nanofibrous matrix for bone tissues engineering
Low temperature fabrication of spherical brushite granules by cement paste emulsion
Small-diameter tissue engineered vascular graft made of electrospun PCL/lecithin blend
Radiation synthesis of PVP/alginate hydrogel containing nanosilver as wound dressing
Preparation of core–shell poly(l-lactic) acid-nanocrystalline apatite hollow microspheres for bone repairing applications
Scaffolds for bone tissue engineering fabricated from two different materials by the rapid prototyping technique: PCL versus PLGA
Seamless, axially aligned, fiber tubes, meshes, microbundles and gradient biomaterial constructs
Polyethylenimine functionalized magnetic nanoparticles as a potential non-viral vector for gene delivery
Electrospun emodin polyvinylpyrrolidone blended nanofibrous membrane: a novel medicated biomaterial for drug delivery and accelerated wound healing
The recombinant human dentin matrix protein 1-coated titanium and its effect on the attachment, proliferation and ALP activity of MG63 cells
The influence of plasma technology coupled to chemical grafting on the cell growth compliance of 3D hydroxyapatite scaffolds
Local delivery of alendronate eluting chitosan scaffold can effectively increase osteoblast functions and inhibit osteoclast differentiation
Effect of testosterone incorporation on cell proliferation and differentiation for polymer–bioceramic composites
Peptide immobilization on polyethylene terephthalate surfaces to study specific endothelial cell adhesion, spreading and migration
Effects of intermittent hydrostatic pressure magnitude on the chondrogenesis of MSCs without biochemical agents under 3D co-culture
Transplantation of nano-bioglass/gelatin scaffold in a non-autogenous setting for bone regeneration in a rabbit ulna
Overcoming hypoxia in 3D culture systems for tissue engineering of bone in vitro using an automated, oxygen-triggered feedback loop
Novel pHEMA-gelatin SPHs as bone scaffolds in dynamic cultures
Laser-engineered topography: correlation between structure dimensions and cell control
Peripheral mineralization of a 3D biodegradable tubular construct as a way to enhance guidance stabilization in spinal cord injury regeneration
Journal of Materials Science: Materials in Medicine : Volume 23, Issue 10, October 2012
Journal of Materials Science: Materials in Medicine : Volume 23, Issue 9, September 2012
Journal of Materials Science: Materials in Medicine : Volume 23, Issue 8, August 2012
Journal of Materials Science: Materials in Medicine : Volume 23, Issue 7, July 2012
Journal of Materials Science: Materials in Medicine : Volume 23, Issue 6, June 2012
Journal of Materials Science: Materials in Medicine : Volume 23, Issue 5, May 2012
Journal of Materials Science: Materials in Medicine : Volume 23, Issue 4, April 2012
Journal of Materials Science: Materials in Medicine : Volume 23, Issue 3, March 2012
Journal of Materials Science: Materials in Medicine : Volume 23, Issue 2, February 2012
Journal of Materials Science: Materials in Medicine : Volume 23, Issue 1, January 2012
Journal of Materials Science: Materials in Medicine : Volume 22
Journal of Materials Science: Materials in Medicine : Volume 21
Journal of Materials Science: Materials in Medicine : Volume 20
Journal of Materials Science: Materials in Medicine : Volume 19
Journal of Materials Science: Materials in Medicine : Volume 18
Journal of Materials Science: Materials in Medicine : Volume 17
Journal of Materials Science: Materials in Medicine : Volume 16
Journal of Materials Science: Materials in Medicine : Volume 15
Journal of Materials Science: Materials in Medicine : Volume 14
Journal of Materials Science: Materials in Medicine : Volume 13
Journal of Materials Science: Materials in Medicine : Volume 12
Journal of Materials Science: Materials in Medicine : Volume 11
Journal of Materials Science: Materials in Medicine : Volume 10
Journal of Materials Science: Materials in Medicine : Volume 9
Journal of Materials Science: Materials in Medicine : Volume 8

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Novel pHEMA-gelatin SPHs as bone scaffolds in dynamic cultures

Content Provider Springer Nature Link
Author Çetin, Damla Kahraman, A. Sera Gümüşderelioğlu, Menemşe
Copyright Year 2012
Abstract The effectiveness of poly(2-hydroxyethyl methacrylate)-gelatin superporous hydrogels (pHEMA-gelatin SPHs) was investigated for bone tissue engineering. The cell culture studies were performed with preosteoblastic MC3T3-E1 cells. Dynamic culture conditions were provided using 100 ml spinner flask rotating at 50 rpm. According to the results of mitochondrial activity test (1-3-[4,5-dimethylthiazol-2-yl]-diphenyltetrazolium bromide), there is no significant difference between proliferation behavior of cells cultured under static and dynamic conditions during 28 days. Observations by scanning electron microscopy and confocal laser scanning microscopy showed that, cells attached well onto the scaffolds and spread through the pores for both culture conditions. However, it was found that, calcium deposition and alkaline phosphatase activity in the scaffolds cultured under dynamic conditions were higher than that of static conditions. The expression of osteogenic differentiation markers, i.e. collagen I and osteopontin, based on real-time reverse transcriptase-polymerase chain reaction demonstrated increased responses under the spinner flask culture conditions. The combination of dynamic culture conditions in spinner flask with the use of superporous pHEMA-gelatin scaffolds enhanced the outcomes related to bone tissue engineering.
Starting Page 2803
Ending Page 2812
Page Count 10
File Format PDF
ISSN 09574530
Journal Journal of Materials Science: Materials in Medicine
Volume Number 23
Issue Number 11
e-ISSN 15734838
Language English
Publisher Springer US
Publisher Date 2012-08-08
Publisher Place Boston
Access Restriction Subscribed
Subject Keyword Polymer Sciences Characterization and Evaluation of Materials Metallic Materials Ceramics, Glass, Composites, Natural Methods Biomaterials Surfaces and Interfaces, Thin Films
Content Type Text
Resource Type Article
Subject Biomaterials Biophysics Bioengineering Biomedical Engineering
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