NDLI: Overcoming hypoxia in 3D culture systems for tissue engineering of bone in vitro using an automated, oxygen-triggered feedback loop

Content Provider	Springer Nature Link
Author	Volkmer, Elias Otto, Sven Polzer, Hans Saller, Maximilian Trappendreher, Daniel Zagar, Darin Hamisch, Sabine Ziegler, Günter Wilhelmi, Arndt Mutschler, Wolf Schieker, Matthias
Copyright Year	2012
Abstract	Tissue engineering is an attractive approach to heal bony defects. However, three-dimensional cell-scaffold constructs display uneven oxygen supply resulting in inhomogeneous tissue quality. We assessed different strategies to improve oxygen supply in vitro. Scaffolds with differing inner surface were seeded with preosteoblastic cells and cultivated either statically or in perfusion bioreactors. Oxygen concentration and pH were measured in the center of the scaffolds. An inductive feedback mechanism was build to increase bioreactor pump speed according to the oxygen concentrations measured within the scaffolds. While pH remained stable, oxygen concentration decreased significantly under static conditions within the cell-seeded scaffolds. Reducing the scaffolds’ inner surface as well as increasing perfusion speeds in bioreactors resulted in improved oxygen supply. We conclude that improving oxygen supply to three dimensional culture systems for bone tissue engineering is feasible in an automated manner. Culture conditions have to be adapted to each cell-scaffold system individually.
Starting Page	2793
Ending Page	2801
Page Count	9
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	23
Issue Number	11
e-ISSN	15734838
Language	English
Publisher	Springer US
Publisher Date	2012-07-29
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Polymer Sciences Characterization and Evaluation of Materials Metallic Materials Ceramics, Glass, Composites, Natural Methods Biomaterials Surfaces and Interfaces, Thin Films
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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