NDLI: Immobilization of a bone and cartilage stimulating peptide to a synthetic bone graft

Content Provider	Springer Nature Link
Author	Wang, Vivian Misra, Gauri Amsden, Brian
Copyright Year	2007
Abstract	A synthetic peptide fragment of human collagen type I (BCSP™-1) was linked to the surface of a commercially available ceramic in an effort to improve the properties of the bone graft substitute to accelerate local healing. BCSP™-1 was covalently immobilized on the surface of the ceramic via the linkers 3-aminopropyl-triethoxysilane (APTES) and suberic acid bis-N-hydroxysuccinimide ester (DSS). The chosen chemistry was non-cytotoxic. A rat calvaria cell assay using alkaline phosphatase (ALP) as an osteoblast differentiation marker, showed that modifying the surface of the ceramic was enough to enhance ALP activity, although the total cell population on the surface decreased. A significant increase in ALP activity/cell was noted with serum albumin bound to the surface, however, the BCSP™-1 bound surface exhibited an even greater ALP activity that showed a surface concentration dependent trend. An optimal BCSP™-1 surface density in the range of 0.87–2.24 nmol/cm$^{2}$ elicited the maximum ALP activity/cell at day 6 of culture. The peptide bound ceramic generated an ALP activity/cell that was roughly 3-fold higher than the non-modified ceramic and 2-fold higher than the APTES-grafted ceramic.
Starting Page	2145
Ending Page	2155
Page Count	11
File Format	PDF
ISSN	09574530
Journal	Journal of Materials Science: Materials in Medicine
Volume Number	19
Issue Number	5
e-ISSN	15734838
Language	English
Publisher	Springer US
Publisher Date	2007-11-22
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Surfaces and Interfaces, Thin Films Ceramics, Glass, Composites, Natural Methods Metallic Materials Polymer Sciences Characterization and Evaluation of Materials Biomaterials
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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