NDLI: Successful intercalation of DNA into CTAB-modified clay minerals for gene protection

Content Provider	Springer Nature Link
Author	Hou, Yakun Wu, Pingxiao Huang, Zhujian Ruan, Bo Liu, Paiyu Zhu, Nengwu
Copyright Year	2014
Abstract	Montmorillonite (MMT) and rectorite (REC) are clay minerals that consist of hydrated aluminum silicate with Si–O tetrahedrons on the bottom of the layer, Al–O(OH)$_{2}$ octahedrons on the top, and various exchangeable ions such as Na$^{+}$ and Ca$^{2+}$ in interlayer. However, the driving force for intercalation of double-stranded DNA is not sufficient to open up the gallery of MMT and REC and allows the intercalation to occur. Furthermore, the external surface of MMT and REC is hydrophilic rather than organophilic. In the study, cationic hexadecyl trimethyl ammonium bromide (CTAB) is chosen to modify MMT and REC to make clay minerals more compatible with DNA. CTAB intercalates into the galleries of clay minerals and expands the basal spacing for DNA intercalation. Novel CTAB-clay/DNA hybrids are synthesized for the first time, with their structure investigated by X-ray diffraction and Fourier transform infrared. Gel electrophoresis analysis confirms that the CTAB-modified clay could protect DNA from degradation of DNase I. UV absorption spectroscopy and circular dichroism indicate that the modified clay minerals can provide a capacity for protecting DNA from damage induced by heavy metals. In addition, the intercalated DNA can be recovered readily under alkaline conditions. Therefore, CTAB-clay/DNA hybrids are potential materials for storage of genetic information.
Starting Page	7273
Ending Page	7281
Page Count	9
File Format	PDF
ISSN	00222461
Journal	Journal of Materials Science
Volume Number	49
Issue Number	20
e-ISSN	15734803
Language	English
Publisher	Springer US
Publisher Date	2014-07-15
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Materials Science Characterization and Evaluation of Materials Polymer Sciences Continuum Mechanics and Mechanics of Materials Crystallography Mechanics
Content Type	Text
Resource Type	Article
Subject	Ceramics and Composites Mechanics of Materials Mechanical Engineering Polymers and Plastics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Preparation and characterization of poly (styrene-acrylonitrile) (SAN)/clay nanocomposites by melt intercalation

Effect of organic intercalation on the viscoelastic behavior of clay

Preparation and characterization of ionic liquid intercalation compounds into layered zirconium phosphates

Superabsorbent polymer composites: does clay always improve properties?

Polymer conversion into amorphous ceramics by ion irradiation

Processing of spodumene-modified mullite ceramics

Intercalation of methylene blue into layered potassium titanoniobate KTiNbO$_{5}$: characterization and electrochemical investigation

Effects of CTAB on porous silica templated by chitosan

Impact of clay nanoparticles on glutaraldehyde crosslinked fiber composites

Successful intercalation of DNA into CTAB-modified clay minerals for gene protection

Similar Documents

Preparation and characterization of poly (styrene-acrylonitrile) (SAN)/clay nanocomposites by melt intercalation

Effect of organic intercalation on the viscoelastic behavior of clay

Preparation and characterization of ionic liquid intercalation compounds into layered zirconium phosphates

Superabsorbent polymer composites: does clay always improve properties?

Polymer conversion into amorphous ceramics by ion irradiation

Processing of spodumene-modified mullite ceramics

Intercalation of methylene blue into layered potassium titanoniobate KTiNbO$_{5}$: characterization and electrochemical investigation

Effects of CTAB on porous silica templated by chitosan

Impact of clay nanoparticles on glutaraldehyde crosslinked fiber composites

Successful intercalation of DNA into CTAB-modified clay minerals for gene protection