NDLI: Analysis of the genome of Azotobacter vinelandii revealed the presence of two genetically distinct group II introns on the chromosome

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Analysis of the genome of Azotobacter vinelandii revealed the presence of two genetically distinct group II introns on the chromosome

Content Provider	Springer Nature Link
Author	Kosaraju, Padma Pulakat, Lakshmi Gavini, Nara
Copyright Year	2005
Abstract	Azotobacter vinelandii belongs to the γ subdivision of eubacteria and has one of the highest respiratory rates. It is considered to be among the probable progenitors of mitochondria. Group II introns were originally identified on organelle genomes. Analysis of the A. vinelandii genome for the presence of group II introns using a deduced group II intron consensus sequence identified two putative introns. The first intron (AV1) which was found to be inserted in the groEL, an essential gene, was already characterized. Our study identified another group II intron (AV2) in A. vinelandii genome. This intron is inserted in a mobile genetic element, similar to most of the group II introns in bacteria, which in this case is a transposase like gene, tnpA1. This putative TnpA1 protein is 52% identical to TnpA, the transposase of bacteriophage Lambda, and 85% identical to TnpA1 of Pseudomonas stutzeri. Sequence analysis showed that this intron encodes a reverse transcriptase (RT) like motif in domain IV, similar to other group II introns. The RT of this intron open reading frame (ORF) is 53% homologous with that of AV1 intron and 66% homologous with that of Pseudomonas putida (Tn5041c) intron. Secondary structure analysis showed that this intron has the typical sub-group IIB1 structure, but the EBS2–IBS2 interaction appears to be missing. Using the RNA generated by in vitro transcription of the intron sequence with its flanking exons, in vitro splicing experiments were performed. It was found that the AV2 intron is functional, despite of lacking the EBS2–IBS2 interaction that plays a role in exon recognition.
Starting Page	107
Ending Page	115
Page Count	9
File Format	PDF
ISSN	00166707
Journal	Genetica
Volume Number	124
Issue Number	2-3
e-ISSN	15736857
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Azotobacter genome groEL group II Introns intron sequence in vitro splicing reverse transcriptase transposase Human Genetics Plant Genetics & Genomics Microbial Genetics and Genomics Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Animal Science and Zoology Insect Science

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Analysis of the genome of Azotobacter vinelandii revealed the presence of two genetically distinct group II introns on the chromosome

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Analysis of the genome of Azotobacter vinelandii revealed the presence of two genetically distinct group II introns on the chromosome