NDLI: Genetic and molecular characterization of Candystripe1 transposition events in sorghum

Content Provider	Springer Nature Link
Author	Carvalho, Carlos H. S. Boddu, Jayanand Zehr, Usha B. Axtell, John D. Pedersen, Jeffery F. Chopra, Surinder
Copyright Year	2005
Abstract	In sorghum, the Candystripe1 (Cs1) transposable element causes a variegated pericarp phenotype due to its excision activity from the y1 (yellow seed1) locus. The Y1 is a transcription regulator which is required for the biosynthesis of red 3-deoxyflavonoid pigments. Somatic variability in the transposition behavior of Cs1 was observed via biochemical analysis of 3-deoxyflavonoids in the leaf tissues of the Y1-cs alleles. Using somatic excisions of Cs1 as a tool, we establish that the Cs1 is active in young seedlings and the y1 locus is also functional in these tissues. Several somatic and germinal excision events were characterized and sequence analysis of independent events predominantly showed 2-bp footprints. Further, with the goal of understanding the properties of Cs1 that would facilitate the development of a transposon tagging system in sorghum, germinal excisions of Cs1 from y1 were used as a marker. Transposition of Cs1 was followed by characterization of putative insertion events. Genetic linkage between mutant phenotypes and the co-segregating restriction fragments of Cs1 provided additional evidence that Cs1 is an active transposable element in sorghum.
Starting Page	201
Ending Page	212
Page Count	12
File Format	PDF
ISSN	00166707
Journal	Genetica
Volume Number	124
Issue Number	2-3
e-ISSN	15736857
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	3-deoxyflavonoids Candystripe1 enhancer/suppressor-mutator phlobaphenes Sorghum bicolor Human Genetics Plant Genetics & Genomics Microbial Genetics and Genomics Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Animal Science and Zoology Insect Science

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