NDLI: Characterization of a vasa homolog in the brown-marbled grouper (Epinephelus fuscoguttatus) and its expression in gonad and germ cells during larval development

Content Provider	Springer Nature Link
Author	Boonanuntanasarn, Surintorn Bunlipatan, Paiboon Ichida, Kensuke Yoohat, Kirana Mengyu, Ornkanya Detsathit, Samart Yazawa, Ryosuke Yoshizaki, Goro
Copyright Year	2016
Abstract	The vasa gene is specifically expressed in the germ cell lineage, and its expression has been used to study germline development in many organisms, including fishes. In this study, we cloned and characterized vasa as Efu-vasa in the brown-marbled grouper (Epinephelus fuscoguttatus). Efu-vasa contained predicted regions that shared consensus motifs with the vasa family in teleosts, including arginine- and glycine-rich repeats, ATPase motifs, and a DEAD box. Phylogenetic-tree construction using various DEAD-box proteins confirmed that Efu-vasa was clustered in the vasa family. Efu-vasa mRNA was detectable only in gonads, by reverse transcription polymerase chain reaction. Primordial germ cells (PGCs) during early gonad development in larvae were characterized by histological examination and in situ hybridization using an Efu-vasa antisense probe. Migrating PGCs were found in larvae at 9–21 days post-hatching, and rapid proliferation of PGCs was initiated in 36 days post-hatching. These findings provide a valuable basis for optimizing the developmental stages for germ cell transplantation in order to produce surrogate broodstock, which may help in the production of larvae of large and endangered grouper species.
Starting Page	1621
Ending Page	1636
Page Count	16
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	42
Issue Number	6
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-07-12
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Brown-marbled grouper Epinephelus fuscoguttatus PGC Primordial germ cell migration Vasa Vasa molecular characterization Freshwater & Marine Ecology Animal Physiology Animal Anatomy / Morphology / Histology Animal Biochemistry Zoology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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