NDLI: Characterization of proteolytic and anti-proteolytic activity involvement in sterlet spermatozoon maturation

Content Provider	Springer Nature Link
Author	Dzyuba, Viktoriya Słowińska, Mariola Cosson, Jacky Ciereszko, Andrzej Boryshpolets, Sergii Štĕrba, Ján Rodina, Marek Linhart, Otomar Dzyuba, Borys
Copyright Year	2016
Abstract	In sturgeon, the acquisition of the potential for motility activation called spermatozoon maturation takes place outside testes. This process can be accomplished in vitro by pre-incubation of immature testicular spermatozoa in seminal fluid collected from fully mature Wolffian duct sperm. Addition of trypsin inhibitor to the pre-incubation medium disrupts spermatozoon maturation. There are no available data for the role of proteolysis regulators in fish spermatozoon maturation, while their role is recognized in mammalian sperm maturation. The present study evaluated the involvement of seminal fluid proteases and anti-proteolytic activity in the sterlet spermatozoon maturation process. Casein and gelatin zymography and quantification of amidase and anti-proteolytic activity were conducted in sturgeon seminal fluid from Wolffian duct sperm and seminal fluid from testicular sperm, along with spermatozoon extracts from Wolffian duct spermatozoa, testicular spermatozoa, and testicular spermatozoa after in vitro maturation. We did not find significant differences in proteolytic profiles of seminal fluids from Wolffian duct sperm and ones from testicular sperm. Zymography revealed differences in spermatozoon extracts: Wolffian duct spermatozoon extracts were characterized by the presence of a broad proteolytic band ranging from 48 to 41 kDa, while testicular spermatozoon extracts did not show such activity until after in vitro maturation. The differences in amidase activity coincided with these results. It may not be the levels of proteolytic and anti-proteolytic activity per se, but the alterations in their interactions triggering a cascade of signaling events, that is crucial to the maturation process.
Starting Page	1755
Ending Page	1766
Page Count	12
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	42
Issue Number	6
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-07-12
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Amidase activity Anti-proteolytic activity Casein and gelatin zymography Siberian sturgeon sperm Spermatozoon maturation Sterlet sperm Freshwater & Marine Ecology Animal Physiology Animal Anatomy / Morphology / Histology Animal Biochemistry Zoology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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