NDLI: Intestinal absorption of amino acids in the Pacific bluefin tuna (Thunnus orientalis): in vitro lysine–arginine interaction using the everted intestine system

Content Provider	Springer Nature Link
Author	Martínez Montaño, Emmanuel Peña, Emyr Viana, María Teresa
Copyright Year	2012
Abstract	The interaction between lysine (Lys) and arginine (Arg) in the proximal intestinal region of Pacific bluefin tuna (Thunnus orientalis) was evaluated using the everted intestine method. This in vitro intestinal system has been shown to be an effective tool for studying the nutrient absorption without the need to handle the tuna fish in marine cages as needed for digestibility and amino acid (AA) absorption. We used a factorial design with two sets of variables: low and high Lys concentration (10 and 75 mM) and four different Arg concentrations (3, 10, 20, and 30 mM). Both amino acids were dissolved in marine Ringer solution with a basal amino acidic composition consisting of a tryptone solution (9 mg mL−1). No interaction was observed between the absorption of Lys and Arg during the first 10 min of the experiment when low concentration of Lys and Arg was used in the hydrolyzate solution. However, there seemed to be a positive effect on Lys absorption when both amino acids were at high concentrations (30 and 75 mM, respectively). This type of studies will led us to test different formulations and/or additives to better understand the efficiency of AA supplementation as an alternative to in situ studies that are difficult to follow to design with the Pacific Bluefin Tuna.
Starting Page	325
Ending Page	334
Page Count	10
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	39
Issue Number	2
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-09-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Amino acid absorption Bluefin tuna Everted intestine In vitro methods Lysine–arginine Interaction Freshwater & Marine Ecology Animal Physiology Animal Anatomy / Morphology / Histology Animal Biochemistry Zoology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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Intestinal absorption of amino acids in the Pacific bluefin tuna (Thunnus orientalis): in vitro lysine-arginine interaction using the everted intestine system.

Visual spectral sensitivity of photopic juvenile Pacific bluefin tuna (Thunnus orientalis)

Characterization and ontogenetic development of digestive enzymes in Pacific bluefin tuna Thunnus orientalis larvae

Development of visual cells in the Pacific bluefin tuna Thunnus orientalis

Changes in the scotopic vision of juvenile Pacific bluefin tuna (Thunnus orientalis) with growth

Retinal ganglion cell topography in juvenile Pacific bluefin tuna Thunnus orientalis (Temminck and Schlegel)

Partial characterization of the digestive enzymes of Pacific bluefin tuna Thunnus orientalis under culture conditions

Molecular characterization of southern bluefin tuna myoglobin (Thunnus maccoyii)

Testes maturation of reared Pacific bluefin tuna Thunnus orientalis at two-plus years old

Intestinal absorption of amino acids in the Pacific bluefin tuna (Thunnus orientalis): in vitro lysine–arginine interaction using the everted intestine system

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Intestinal absorption of amino acids in the Pacific bluefin tuna (Thunnus orientalis): in vitro lysine-arginine interaction using the everted intestine system.

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Intestinal absorption of amino acids in the Pacific bluefin tuna (Thunnus orientalis): in vitro lysine–arginine interaction using the everted intestine system