NDLI: Characterization and ontogenetic development of digestive enzymes in Pacific bluefin tuna Thunnus orientalis larvae

Content Provider	Springer Nature Link
Author	Murashita, Koji Matsunari, Hiroyuki Kumon, Kazuri Tanaka, Yosuke Shiozawa, Satoshi Furuita, Hirofumi Oku, Hiromi Yamamoto, Takeshi
Copyright Year	2014
Abstract	The major digestive enzymes in Pacific bluefin tuna Thunnus orientalis larvae were characterized, and the physiological characteristics of the enzymes during early ontogeny were clarified using biochemical and molecular approaches. The maximum activity of trypsin (Try), chymotrypsin (Ct) and amylase (Amy) was observed at pH 6–11, 8–11 and 6–9, respectively. Maximum activity of Try, Ct and Amy occurred at 50 °C, that of lipase (Lip) was at 60 °C and that of pepsin (Pep) was at 40–50 °C. These pH and thermal profiles were similar to those for other fish species but differed from those previously reported for adult bluefin tuna. Enzyme activity for all enzymes assayed was found to decrease at high temperatures (Try, Ct, Amy and Pep: 50 °C; Lip: 40 °C), which is similar to findings for other fish species with one marked exception—increased Try activity was observed at 40 °C. Lip activity appeared to be dependent on bile salts under our assay conditions, resulting in a significant increase in activity in the presence of bile salts. Ontogenetic changes in pancreatic digestive enzymes showed similar gene expression patterns to those of other fish species, whereas marked temporal increases in enzyme activities were observed at 10–12 days post hatching (dph), coinciding with previously reported timing of the development of the pyloric caeca in bluefin tuna larvae. However, complete development of digestive function was indicated by the high pep gene expression from 19 dph, which contradicts the profile of Pep activity and previously reported development timing of the gastric gland. These findings contribute to the general knowledge of bluefin tuna larval digestive system development.
Starting Page	1741
Ending Page	1755
Page Count	15
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	40
Issue Number	6
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-07-24
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Pacific bluefin tuna Ontogenetic development Digestive enzyme Activity Gene expression Freshwater & Marine Ecology Animal Physiology Animal Anatomy / Morphology / Histology Animal Biochemistry Zoology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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