NDLI: Isolation of heat-tolerant myoglobin from Asian swamp eel Monopterus albus

Content Provider	Springer Nature Link
Author	Chotichayapong, Chatrachatchaya Wiengsamut, Kittipong Chanthai, Saksit Sattayasai, Nison Tamiya, Toru Kanzawa, buyuki Tsuchiya, Takahide
Copyright Year	2012
Abstract	Myoglobin from Asian swamp eel Monopterus albus was purified from fish muscle using salt fractionation followed by column chromatography and molecular filtration. The purified Mb of 0.68 mg/g wet weight of muscle was determined for its molecular mass by MALDI-TOF–MS to be 15,525.18 Da. Using isoelectric focusing technique, the purified Mb showed two derivatives with pI of 6.40 and 7.12. Six peptide fragments of this protein identified by LC–MS/MS were homologous to Mbs of sea raven Hemitripterus americanus, yellowfin tuna Thunnus albacores, blue marlin Makaira nigicans, common carp Cyprinus carpio, and goldfish Carassius auratus. According to the Mb denaturation, the swamp eel Mb had thermal stability higher than walking catfish Clarias batrachus Mb and striped catfish Pangasius hypophthalmus Mb, between 30 and 60 °C. For the thermal stability of Mb, the swamp eel Mb showed a biphasic behavior due to the O2 dissociation and the heme orientation disorder, with the lowest increase in both Kdf and Kds. The thermal sensitivity of swamp eel Mb was lower than those of the other Mbs for both of fast and slow reaction stages. These results suggest that the swamp eel Mb globin structure is thermally stable, which is consistent with heat-tolerant behavior of the swamp eel particularly in drought habitat.
Starting Page	1533
Ending Page	1543
Page Count	11
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	38
Issue Number	5
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-04-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Denaturation rate LC–MS/MS MALDI-TOF–MS Myoglobin Peptide fragment Swamp eel Monopterus albus Thermal stability Animal Anatomy / Morphology / Histology Zoology Animal Biochemistry Animal Physiology Freshwater & Marine Ecology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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