NDLI: Purification and characterization of pepsinogens and pepsins from the stomach of rice field eel (Monopterus albus Zuiew)

Content Provider	Springer Nature Link
Author	Weng, Wu Yin Wu, Tao Chen, Wei Qin Liu, Guang Ming Osatomi, Kiyoshi Su, Wen Jin Cao, Min Jie
Copyright Year	2010
Abstract	Three pepsinogens (PG1, PG2, and PG3) were highly purified from the stomach of freshwater fish rice field eel (Monopterus albus Zuiew) by ammonium sulfate fractionation and chromatographies on DEAE-Sephacel, Sephacryl S-200 HR. The molecular masses of the three purified PGs were all estimated as 36 kDa using SDS–PAGE. Two-dimensional gel electrophoresis (2D-PAGE) showed that pI values of the three PGs were 5.1, 4.8, and 4.6, respectively. All the PGs converted into corresponding pepsins quickly at pH 2.0, and their activities could be specifically inhibited by aspartic proteinase inhibitor pepstatin A. Optimum pH and temperature of the enzymes for hydrolyzing hemoglobin were 3.0–3.5 and 40–45°C. The K m values of them were 1.2 × 10−4 M, 8.7 × 10−5 M, and 6.9 × 10−5 M, respectively. The turnover numbers (k cat) of them were 23.2, 24.0, and 42.6 s−1. Purified pepsins were effective in the degradation of fish muscular proteins, suggesting their digestive functions physiologically.
Starting Page	543
Ending Page	552
Page Count	10
File Format	PDF
ISSN	09201742
Journal	Fish Physiology and Biochemistry
Volume Number	37
Issue Number	3
e-ISSN	15735168
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-12-08
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Rice field eel Purification Pepsinogen Pepsin Western blot Freshwater & Marine Ecology Animal Physiology Zoology Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry Aquatic Science

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