NDLI: Doubling the chromosome number of bahiagrass via tissue culture

Content Provider	Springer Nature Link
Author	Quesenberry, Kenneth H. Dampier, Judith M. Lee, Y. Y. Smith, Rex L. Acuña, Carlos A.
Copyright Year	2010
Abstract	Crop improvement in bahiagrass (Paspalum notatum Flüggé) is limited by apomixis in most natural tetraploids, however, diploid sexual types occur. Production of sexual tetraploids by chromosome doubling will allow hybridization with apomictic tetraploids. Diploid bahiagrass (Paspalum notatum Flüggé) embryogenic callus tissue was exposed to three concentrations of three antimitotic chemical agents, colchicine, oryzalin and trifluralin. Callus was generated to plants and ploidy was evaluated by stomata size, mitotic chromosome counts, and flow cytometry. A total of 310 plants were verified as tetraploid of 1,432 plants that reached transplanting size. All treatments yielded 4x plants. The mean percentage success over all treatments was 22%, with means of 31% for oryzalin, 24% for colchicine and 16% for trifluralin. The high rates of success indicate that all agents can be successfully used to double chromosome numbers in bahiagrass. The percentage of 4x plants ranged from 9% (20 μM trifluralin) to 43% (20 μM oryzalin). Several treatments adversely affected regeneration. Mitotic chromosome counts are difficult and labor intensive in bahiagrass. Therefore, leaf stomata measurements were used as a preliminary screen. Data gave a bimodal distribution with overlapping tails and based on chromosome counts would have given an error rate of 12%. Flow cytometry analysis of regenerated plants resulted in mean nucleus fluorescence distributions consistent with control diploid or tetraploid values. These values agreed with chromosome counts, and this method is recommended for determining bahiagrass ploidy level. Research goals and available resources should be taken into consideration when selecting a treatment for chromosome doubling in bahiagrass.
Starting Page	43
Ending Page	50
Page Count	8
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	175
Issue Number	1
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-04-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Polyploidy Chromosome doubling Paspalum Tissue culture Biotechnology Plant Physiology Plant Pathology Plant Genetics & Genomics Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Tetraploid bahiagrass hybrids: breeding technique, genetic variability and proportion of heterotic hybrids

Agronomic characterization of autotetraploid banana plants derived from ‘Pisang Lilin’ (AA) obtained through chromosome doubling

Development of triploid daylily (Hemerocallis) germplasm by embryo rescue

Induction of 2n pollen with colchicine during microsporogenesis in Eucalyptus

In vitro chemical mutagenesis improves the turf quality of bahiagrass

Genetics of some in vitro characters in pearl millet

Utilization of crop heterosis: a review

Mitotic chromosome doubling of plant tissues in vitro

In vitro-induced tetraploids of Plectranthus esculentus are nematode-tolerant and have enhanced nutritional value

Doubling the chromosome number of bahiagrass via tissue culture

Similar Documents

Tetraploid bahiagrass hybrids: breeding technique, genetic variability and proportion of heterotic hybrids

Agronomic characterization of autotetraploid banana plants derived from ‘Pisang Lilin’ (AA) obtained through chromosome doubling

Development of triploid daylily (Hemerocallis) germplasm by embryo rescue

Induction of 2n pollen with colchicine during microsporogenesis in Eucalyptus

In vitro chemical mutagenesis improves the turf quality of bahiagrass

Genetics of some in vitro characters in pearl millet

Utilization of crop heterosis: a review

Mitotic chromosome doubling of plant tissues in vitro

In vitro-induced tetraploids of Plectranthus esculentus are nematode-tolerant and have enhanced nutritional value

Doubling the chromosome number of bahiagrass via tissue culture