NDLI: Identification and sequence characterisation of molecular markers polymorphic between male kiwifruit (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev.) accessions exhibiting different flowering time

Content Provider	Springer Nature Link
Author	vo, Marina Romo, Silvia Rey, Manuel Prado, María Jesús González, María Victoria
Copyright Year	2010
Abstract	Fruit set in kiwifruit is strongly dependent on pollination, which is limited by the lack of efficient male pollen donors, among other factors. We searched for molecular markers that could be polymorphic in relation to flowering time in order to classify male kiwifruit plants to discard those that are not likely to perform as efficient pollen donors. Random amplified polymorphic DNA (RAPD) and modified amplified fragment length polymorphism (AFLP) markers were generated using 41 male kiwifruit plants in two flowering groups, early- and late-flowering males (with respect to the female cultivar ‘Hayward’). One RAPD and nine modified-AFLP markers polymorphic between male plants exhibiting different flowering time were identified, sequenced and analysed in databases. Unweighted pair group method with arithmetic average (UPGMA) clustering and multidimensional scaling showed that these markers could be used to classify the male plants into flowering groups. Analysis of molecular variance (AMOVA) agreed with this classification, showing that most of the genetic variation is found between flowering groups. Sequence analysis based on a database search revealed that the polymorphism PolM contains a 7-nucleotide long element involved in the repression of the phytochrome A gene, that Pol4 is a partial sequence of a phytochrome B gene, and that sequences Pol3, Pol5, Pol7, and Pol9 show high identity with ESTs from kiwifruit buds treated with hydrogen cyanamide. Clustering analysis supported the previous classification of males into flowering groups, making it feasible to predict male plants’ flowering times with respect to the cultivar ‘Hayward’ based upon these molecular markers.
Starting Page	109
Ending Page	121
Page Count	13
File Format	PDF
ISSN	00142336
Journal	Euphytica
Volume Number	175
Issue Number	1
e-ISSN	15735060
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-05-27
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Kiwifruit Flowering time Molecular markers RAPD Modified-AFLP Marker assisted selection Biotechnology Plant Physiology Plant Pathology Plant Genetics & Genomics Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Horticulture Agronomy and Crop Science

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