NDLI: Microelectrophoretic study of environmentally induced DNA damage in fish and its use for early toxicity screening of freshwater bodies

Content Provider	Springer Nature Link
Author	Hussain, Bilal Sultana, Tayyaba Sultana, Salma Al Ghanim, K. A. Masood, Shahreef Ali, Muhammad Mahboob, Shahid
Copyright Year	2017
Abstract	This study investigates the potential of the comet and micronucleus assays of fish DNA as a means of screening the toxicity of aquatic environments. Catla catla and Cirrhinus mrigala collected from the River Chenab in Pakistan were used as a case study for the application of comet and micronucleus techniques. Comet and micronucleus assays were used to compare DNA damage in C. catla and C. mrigala collected from polluted areas of the River Chenab and farmed fish. Atomic absorption spectrophotometry showed an acute level of toxicity from Cd, Cu, Mn, Zn, Pb, Cr, Sn, and Hg in river water. Comet assay showed significant (p < 0.05) DNA damage in C. catla representing 17.33 ± 2.42, 11.53 ± 2.14, and 14.17% DNA in the comet tail, averaged from three sites of the polluted area of the river. Tail moment was observed as 10.06 ± 2.71, 3.11 ± 0.74, and 14.70 ± 1.89, while olive moment was 8.85 ± 1.84, 3.83 ± 0.76, and 7.11 ± 0.73, respectively. Highly significant (p < 0.01) damage was reported in C. mrigala as 37.29 ± 2.51, 34.96 ± 2.53, and 38.80 ± 2.42% DNA in comet tail, tail moment was 23.48 ± 3.90, 19.78 ± 4.26, and 14.30 ± 1.82, and olive moment was 16.22 ± 2.04, 13.83 ± 1.96, and10.99 ± 0.90. Significant (p < 0.05) differences were observed in genotoxicity between farmed and polluted area fish. Micronucleus assay showed a similar picture of significant difference in respect to single and double micronucleus induction: i.e., 23.20 ± 4.19 and 2.80 ± 1.07‰ in C. catla and 44.80 ± 3.73 and 06.20 ± 0.97‰, respectively, in C. mrigala. Nuclear abnormalities were found as 6.00 ± 0.84 and 09.60 ± 1.72/thousand cells, respectively, in both species. The results of this study suggest that these novel fish DNA damage assays can be used as an expedient toxicity screening for aquatic environments.
Starting Page	1
Ending Page	11
Page Count	11
File Format	PDF
ISSN	01676369
Journal	Environmental Monitoring and Assessment
Volume Number	189
Issue Number	3
e-ISSN	15732959
Language	English
Publisher	Springer International Publishing
Publisher Date	2017-02-17
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	DNA fragmentation Biomarker Indian major carps Genotoxicity Monitoring/Environmental Analysis Environmental Management Ecotoxicology Atmospheric Protection/Air Quality Control/Air Pollution Ecology
Content Type	Text
Resource Type	Article
Subject	Pollution Medicine Management, Monitoring, Policy and Law

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