NDLI: Diversity of crude oil-degrading bacteria and alkane hydroxylase (alkB) genes from the Qinghai-Tibet Plateau

Content Provider	Springer Nature Link
Author	Long, Haozhi Wang, Yilin Chang, Sijing Liu, Guangxiu Chen, Tuo Huo, Guanghua Zhang, Wei Wu, Xiukun Tai, Xisheng Sun, Likun Zhang, Baogui
Copyright Year	2017
Abstract	The aim of this study was to survey the response of the microbial community to crude oil and the diversity of alkane hydroxylase (alkB) genes in soil samples from the Qinghai-Tibet Plateau (QTP). The enrichment cultures and clone libraries were used. Finally, 53 isolates and 94 alkB sequences were obtained from 10 pristine soil samples after enrichment at 10 °C with crude oil as sole carbon source. The isolates fell into the phyla Proteobacteria, Actinobacteria, and Bacteroidetes, with the dominance of Pseudomonas and Acinetobacter. The composition of degraders was different from polar habitats where Acinetobacter sp. is not a predominant responder of alkane degradative microbial communities. Phylogenetic analysis showed that the alkB genes from isolates and enrichment communities formed eight clusters and mainly related with alkB genes of Pseudomonas, Rhodococcus, and Acinetobacter. The alkB gene diversity in the QTP was lower than marine environments and polar soil samples. In particular, a total of 10 isolates exhibiting vigorous growth with crude oil could detect no crude oil degradation-related gene sequences, such as alkB, P450, almA, ndoB, and xylE genes. The Shannon-Wiener index of the alkB clone libraries from the QTP ranged from 1.00 to 2.24 which is similar with polar pristine soil samples but lower than that of contaminated soils. These results indicated that the Pseudomonas, Acinetobacter, and Rhodococcus genera are the candidate for in situ bioremediation, and the environment of QTP may be still relatively uncontaminated by crude oil.
Starting Page	1
Ending Page	14
Page Count	14
File Format	PDF
ISSN	01676369
Journal	Environmental Monitoring and Assessment
Volume Number	189
Issue Number	3
e-ISSN	15732959
Language	English
Publisher	Springer International Publishing
Publisher Date	2017-02-20
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Qinghai-Tibet Plateau Crude oil enrichment Alkane hydrolase gene Diversity Monitoring/Environmental Analysis Environmental Management Ecotoxicology Atmospheric Protection/Air Quality Control/Air Pollution Ecology
Content Type	Text
Resource Type	Article
Subject	Pollution Medicine Management, Monitoring, Policy and Law

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