NDLI: Stability of lysosomal membrane in Carcinus maenas acts as a biomarker of exposure to pharmaceuticals

Content Provider	Springer Nature Link
Author	Aguirre Martínez, G. V. Buratti, S. Fabbri, E. Valls, T. A. Martín Díaz, M. L.
Copyright Year	2012
Abstract	The presence of pharmaceuticals in the environment is now a major concern given their potential adverse effects on organisms, particularly human beings. Because the feeding style and habitat of the crab Carcinus maenas make this species vulnerable to organic contaminants, it has been used previously in ecotoxicological studies. Lysosomal membrane stability (LMS) in crabs is a general indicator of cellular well-being and can be visualized by the neutral red retention (NRR) assay. LMS in crab hemolymph has been evaluated as a cellular biomarker of adverse effects produced by exposure to pharmaceutical compounds. Crabs were exposed in the laboratory to four different pharmaceuticals for 28 days in a semistatic 24-h renewal assay. Filtered seawater was spiked every 2 days with various concentrations (from 0.1 to 50 μg·L−1) of caffeine, ibuprofen, carbamazepine, and novobiocin. Results showed that NRR time, measured at day 28, was significantly reduced (p < 0.05) after exposure to environmental concentrations of each pharmaceutical (caffeine = 15 μg·L−1; carbamazepine = 1 μg·L−1; ibuprofen = 5 μg·L−1; and novobiocin = 0.1 μg·L−1) when compared with control organisms. The predicted “no environmental effect” concentration/measured environmental concentration results showed that the selected pharmaceuticals are toxic at environmental concentrations and need further assessment. LMS monitoring in crabs is a sensitive tool for evaluating exposure to concentrations of selected drugs under laboratory conditions and provides a robust tier 1 testing approach (screening biomarker) for rapid assessment of marine pollution and environmental impact assessments for analyzing pharmaceutical contamination in aquatic environments.
Starting Page	3783
Ending Page	3793
Page Count	11
File Format	PDF
ISSN	01676369
Journal	Environmental Monitoring and Assessment
Volume Number	185
Issue Number	5
e-ISSN	15732959
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-11-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hemolymph Neutral red Bioassay Therapeutic drugs Crabs Environmental Monitoring/Analysis Environmental Management Ecotoxicology Atmospheric Protection/Air Quality Control/Air Pollution Ecology
Content Type	Text
Resource Type	Article
Subject	Pollution Medicine Management, Monitoring, Policy and Law

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