NDLI: Monitoring of bioaerosol inhalation risks in different environments using a six-stage Andersen sampler and the PCR-DGGE method

Content Provider	Springer Nature Link
Author	Xu, Zhenqiang Yao, Maosheng
Copyright Year	2012
Abstract	Increasing evidences show that inhalation of indoor bioaerosols has caused numerous adverse health effects and diseases. However, the bioaerosol size distribution, composition, and concentration level, representing different inhalation risks, could vary with different living environments. The six-stage Andersen sampler is designed to simulate the sampling of different human lung regions. Here, the sampler was used in investigating the bioaerosol exposure in six different environments (student dorm, hospital, laboratory, hotel room, dining hall, and outdoor environment) in Beijing. During the sampling, the Andersen sampler was operated for 30 min for each sample, and three independent experiments were performed for each of the environments. The air samples collected onto each of the six stages of the sampler were incubated on agar plates directly at 26 °C, and the colony forming units (CFU) were manually counted and statistically corrected. In addition, the developed CFUs were washed off the agar plates and subjected to polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) for diversity analysis. Results revealed that for most environments investigated, the culturable bacterial aerosol concentrations were higher than those of culturable fungal aerosols. The culturable bacterial and fungal aerosol fractions, concentration, size distribution, and diversity were shown to vary significantly with the sampling environments. PCR-DGGE analysis indicated that different environments had different culturable bacterial aerosol compositions as revealed by distinct gel band patterns. For most environments tested, larger (>3 μm) culturable bacterial aerosols with a skewed size distribution were shown to prevail, accounting for more than 60 %, while for culturable fungal aerosols with a normal size distribution, those 2.1–4.7 μm dominated, accounting for 20–40 %. Alternaria, Cladosporium, Chaetomium, and Aspergillus were found abundant in most environments studied here. Viable microbial load per unit of particulate matter was also shown to vary significantly with the sampling environments. The results from this study suggested that different environments even with similar levels of total microbial cuturable aerosol concentrations could present different inhalation risks due to different bioaerosol particle size distribution and composition. This work fills literature gaps regarding bioaerosol size and composition-based exposure risks in different human dwellings in contrast to a vast body of total bioaerosol levels.
Starting Page	3993
Ending Page	4003
Page Count	11
File Format	PDF
ISSN	01676369
Journal	Environmental Monitoring and Assessment
Volume Number	185
Issue Number	5
e-ISSN	15732959
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-09-07
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Six-stage Andersen sampler Bacterial and fungal aerosols Diversity Inhalation risks Environmental Monitoring/Analysis Environmental Management Ecotoxicology Atmospheric Protection/Air Quality Control/Air Pollution Ecology
Content Type	Text
Resource Type	Article
Subject	Pollution Medicine Management, Monitoring, Policy and Law

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Bioaerosols as contributors to poor air quality in Taichung City, Taiwan

Applicability of a modified MCE filter method with Button Inhalable Sampler for monitoring personal bioaerosol inhalation exposure

Effects of fomesafen on soil enzyme activity, microbial population, and bacterial community composition

Effects of lead and zinc mining contamination on bacterial community diversity and enzyme activities of vicinal cropland

Evaluation of applicability of the Sartorius Airport MD8 sampler for detection of Bacillus endospores in indoor air

Responses of soil fungal community to the sandy grassland restoration in Horqin Sandy Land, northern China

Extraction of the metagenomic DNA and assessment of the bacterial diversity from the petroleum-polluted sites

Bacterial community composition of sediments from Artificial Lake Maslak, Istanbul, Turkey

Impact of water quality on bacterioplankton assemblage along Cértima River Basin (central western Portugal) assessed by PCR–DGGE and multivariate analysis

Monitoring of bioaerosol inhalation risks in different environments using a six-stage Andersen sampler and the PCR-DGGE method

Similar Documents

Bioaerosols as contributors to poor air quality in Taichung City, Taiwan

Applicability of a modified MCE filter method with Button Inhalable Sampler for monitoring personal bioaerosol inhalation exposure

Effects of fomesafen on soil enzyme activity, microbial population, and bacterial community composition

Effects of lead and zinc mining contamination on bacterial community diversity and enzyme activities of vicinal cropland

Evaluation of applicability of the Sartorius Airport MD8 sampler for detection of Bacillus endospores in indoor air

Responses of soil fungal community to the sandy grassland restoration in Horqin Sandy Land, northern China

Extraction of the metagenomic DNA and assessment of the bacterial diversity from the petroleum-polluted sites

Bacterial community composition of sediments from Artificial Lake Maslak, Istanbul, Turkey

Impact of water quality on bacterioplankton assemblage along Cértima River Basin (central western Portugal) assessed by PCR–DGGE and multivariate analysis

Monitoring of bioaerosol inhalation risks in different environments using a six-stage Andersen sampler and the PCR-DGGE method