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| Content Provider | Springer Nature Link |
|---|---|
| Author | Raje, Maj Dhiman, Rohan Majumdar, Sekhar Dass, Taposh Dikshit, Kanak L. Kaur, Ramandeep |
| Copyright Year | 2006 |
| Abstract | Preparation of isolated cells and microorganisms for ultrastructural examination always provides a challenge in terms of adequate immobilization of the cells and prevention of subsequent sample loss and damage during various steps of sample processing. Using a positively charged nylon membrane substrate we demonstrate that it is possible to easily immobilize and retain a sample of isolated cells in culture for a wide variety of microscopy-based techniques. Radiolabelled E. coli cells when immobilized on the charged membrane were seen to be highly resistant to detachment when subjected to the normal sample processing procedures associated with microscopy. In contrast cells on regular millipore membranes were rapidly lost during sample preparation. We demonstrate the utility of charged nylon membranes for a wide variety of microscopy based analysis including scanning and transmission electron microscopy (SEM and TEM), atomic force microscopy (AFM), TEM based immunogold labelling, laser confocal microscopy and SEM based elemental analysis. |
| Starting Page | 111 |
| Ending Page | 117 |
| Page Count | 7 |
| File Format | |
| ISSN | 09209069 |
| Journal | Cytotechnology |
| Volume Number | 51 |
| Issue Number | 2 |
| e-ISSN | 15730778 |
| Language | English |
| Publisher | Kluwer Academic Publishers |
| Publisher Date | 2006-11-03 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Charged membrane Electron microscopy Processing Immobilization Isolated cells Biochemistry Biomedicine general Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology |
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