NDLI: Approach toward an efficient inoculum preparation stage for suspension BHK-21 cell culture

Content Provider	Springer Nature Link
Author	Fernández Núñez, Eutimio Gustavo Leme, Jaci Almeida Parizotto, Letícia Rezende, Alexandre Gonçalves Costa, Bru Labate Vale Boldorini, Vera Lucia Lopes Jorge, Soraia Attie Calil Astray, Renato Mancini Pereira, Carlos Augusto Caricati, Celso Pereira Tonso, Aldo
Copyright Year	2014
Abstract	Mammalian cells are the most frequently used hosts for biopharmaceutical proteins manufacturing. Inoculum quality is a key element for establishing an efficient bioconversion process. The main objective in inoculation expansion process is to generate large volume of viable cells in the shortest time. The aim of this paper was to optimize the inoculum preparation stage of baby hamster kidney (BHK)-21 cells for suspension cultures in benchtop bioreactors, by means of a combination of static and agitated culture systems. Critical parameters for static (liquid column height: 5, 10, 15 mm) and agitated (working volume: 35, 50, 65 mL, inoculum volume percentage: 10, 30 % and agitation speed: 25, 60 rpm) cultures were study in T-flask and spinner flask, respectively. The optimal liquid column height was 5 mm for static culture. The maximum viable cell concentration in spinner flask cultures was reached with 50 mL working volume and the inoculum volume percentage was not significant in the range under study (10–30 %) at 25 rpm agitation. Agitation speed at 60 rpm did not change the main kinetic parameters with respect to those observed for 25 rpm. These results allowed for a schedule to produce more than 4 × 109 BHK-21 cells from 4 × 106 cells in 13 day with 1,051 mL culture medium.
Starting Page	95
Ending Page	104
Page Count	10
File Format	PDF
ISSN	09209069
Journal	Cytotechnology
Volume Number	68
Issue Number	1
e-ISSN	15730778
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-06-19
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Inoculum preparation Mammalian cells BHK-21 Static cell culture Suspension cell culture Spinner flask Bioreactor Biotechnology Biomedicine general Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

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