NDLI: Comparison of different culture modes for long-term expansion of neural stem cells

Content Provider	Springer Nature Link
Author	Zheng, Ke Liu, Tian Qing Dai, Ming Shu Ge, Dan Li, Xiang Qin Ma, Xue Hu Cui, Zhan Feng
Copyright Year	2006
Abstract	Neural stem cells (NSCs) can be cultured in two modes of suspension and monolayer in vitro. The cultured cells are different in both the ability to proliferate and heterogeneity. In order to find the appropriate methods for large-scale expansion of NSCs, we systematically compared the NSCs cultured in suspension with those cultured in monolayer. The forebrain tissue was removed from embryonic day 14 (E14) mice, then the tissue was dissociated into single-cell suspension by Accutase and mechanical trituration. The cells were cultured in both suspension and monolayer. The NSCs cultured in suspension and in monolayer were compared on viability, ability to proliferate and heterogeneity by fluorescent dyes, immunofluorescence and flow cytometry on DIV21 (21 days in vitro), DIV56 and DIV112, respectively. The results indicated that the NSCs cultured in both suspension and monolayer represented good viability in long-term cultures. But they displayed a distinct ability to proliferate in long-term cultures. The NSCs cultured in monolayer preceded those cultured in suspension on the ability to proliferate on DIV21 and DIV56, but no obvious difference on DIV112. The NSCs population cultured in suspension displayed more nestin-positive cells than those in monolayer during the whole process of culture. The NSCs population cultured in monolayer, however, displayed more βIII tubulin-positive cells than those in suspension in the same period. The suspension culture mode excels the monolayer culture mode for large-scale expansion of NSCs.
Starting Page	209
Ending Page	218
Page Count	10
File Format	PDF
ISSN	09209069
Journal	Cytotechnology
Volume Number	52
Issue Number	3
e-ISSN	15730778
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-12-19
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cell culture Monolayer Neural stem cells Neurospheres Suspension Biochemistry Biomedicine general Biotechnology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

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