NDLI: Isolation and characterization of eight polymorphic microsatellite loci from Ludwigia polycarpa (Onagraceae), a threaten herb in North America

Content Provider	Springer Nature Link
Author	Huang, Chi Chun Chiang, Tzen Yuh Hsu, Tsai Wen Hung, Cheng Yu Chiang, Yu Chung Hung, Kuo Hsiang
Copyright Year	2008
Abstract	Ludwigia polycarpa is the only species of section Microcarpium occurring north of 37°N in the central midwest of the United States. Recently, the loss of wetlands in North America reduced the population number and size of L. polycarpa dramatically. In this study, for the purpose of the conservation of the endangered species, we described eight microsatellite DNA loci. High variabilities enable these molecular markers to assess the population structure. The number of alleles per locus ranged from 8 to 23. The expected (H E) and observed (H O) heterozygosities ranged from 0.86 to 0.96 and 0.00 to 1.00, respectively. Seven of the eight microsatellite loci displayed significant departures from Hardy–Weinberg expectations, likely due to the loss of habitats and the small population size. No linkage disequilibrium was observed in the pairwise comparisons of loci. The application of these microsatellite loci in L. polycarpa may provide a tool for understanding its demography and population structure.
Starting Page	1381
Ending Page	1383
Page Count	3
File Format	PDF
ISSN	15660621
Journal	Conservation Genetics
Volume Number	10
Issue Number	5
e-ISSN	15729737
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-08-28
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Conservation Ludwigia polycarpa Microsatellite PIMA RAPD-PCR enrichment Plant Sciences Evolutionary Biology Conservation Biology/Ecology Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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