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Squid Giant Axon Contains Neurofilament Protein mRNA but does not Synthesize Neurofilament Proteins

Content Provider	Springer Nature Link
Author	Gainer, Harold House, Shirley Kim, Dong Sun Chin, Hemin Pant, Harish C.
Copyright Year	2016
Abstract	When isolated squid giant axons are incubated in radioactive amino acids, abundant newly synthesized proteins are found in the axoplasm. These proteins are translated in the adaxonal Schwann cells and subsequently transferred into the giant axon. The question as to whether any de novo protein synthesis occurs in the giant axon itself is difficult to resolve because the small contribution of the proteins possibly synthesized intra-axonally is not easily distinguished from the large amounts of the proteins being supplied from the Schwann cells. In this paper, we reexamine this issue by studying the synthesis of endogenous neurofilament (NF) proteins in the axon. Our laboratory previously showed that NF mRNA and protein are present in the squid giant axon, but not in the surrounding adaxonal glia. Therefore, if the isolated squid axon could be shown to contain newly synthesized NF protein de novo, it could not arise from the adaxonal glia. The results of experiments in this paper show that abundant 3H-labeled NF protein is synthesized in the squid giant fiber lobe containing the giant axon’s neuronal cell bodies, but despite the presence of NF mRNA in the giant axon no labeled NF protein is detected in the giant axon. This lends support to the glia–axon protein transfer hypothesis which posits that the squid giant axon obtains newly synthesized protein by Schwann cell transfer and not through intra-axonal protein synthesis, and further suggests that the NF mRNA in the axon is in a translationally repressed state.
Starting Page	475
Ending Page	486
Page Count	12
File Format	PDF
ISSN	02724340
Journal	Cellular and Molecular Neurobiology
Volume Number	37
Issue Number	3
e-ISSN	15736830
Language	English
Publisher	Springer US
Publisher Date	2016-05-20
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Squid axon Neurofilament protein Local protein synthesis mRNA Ribonuclease protection Schwann cell Neurosciences Cell Biology Neurobiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cellular and Molecular Neuroscience

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